Identification of parasitoid species using PCR amplification and restriction enzyme digestion

Détails

ID Serval
serval:BIB_F790BC563DD3
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Titre
Identification of parasitoid species using PCR amplification and restriction enzyme digestion
Titre de la conférence
XIV International Entomophagous Insects Workshop: June 11-15, 2006, Newark, Delaware
Auteur(s)
Jourdie V., Alvarez N., Turlings T.
ISBN
1536-2442
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Editeur scientifique
Hopper K.R., Kester K.M., Hoelmer K.A.
Volume
7
Série
Journal of Insect Science
Pages
12
Langue
anglais
Résumé
Spodoptera frugiperda is a pest of great economic importance in the Americas. It is attacked by several species of parasitoids, which act as biological control agents. Parasitoids are morphologically identifiable as adults, but not as larvae. Laboratory rearing conditions are not always optimal to rear out parasitic wasps from S. frugiperda larvae collected from wild populations, and it frequently happens that parasitoids do not complete their life cycle and stop developing at the larval stage. Therefore, we explored ways to identify parasitoid larvae using molecular techniques. Sequencing is one possible technique, yet it is expensive. Here we present an alternate, cheaper way of identifying seven species of parasitoids (Cotesia marginiventris, Campoletis sonorensis, Pristomerus spinator, Chelonus insularis, Chelonus cautus, Eiphosoma vitticolle and Meteorus laphygmae) using PCR amplification of COI gene followed by a digestion with a combination of four restriction endonucleases. Each species was found to exhibit a specific pattern when the amplification product was run on an agarose gel. Identifying larvae revealed that conclusions on species composition of a population of parasitic wasps can be biased if only the emerging adults are taken into account.
Web of science
Open Access
Oui
Création de la notice
18/04/2010 12:27
Dernière modification de la notice
09/05/2019 3:38
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