Quantitation and clonal isolation of cytolytic T lymphocyte precursors selectively infiltrating murine sarcoma virus-induced tumors

Détails

ID Serval
serval:BIB_F7716FC0566E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Quantitation and clonal isolation of cytolytic T lymphocyte precursors selectively infiltrating murine sarcoma virus-induced tumors
Périodique
Journal of Experimental Medicine
Auteur(s)
Brunner  K. T., MacDonald  H. R., Cerottini  J. C.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
08/1981
Volume
154
Numéro
2
Pages
362-73
Notes
Journal Article --- Old month value: Aug 1
Résumé
A limiting dilution mixed leukocyte-tumor cell microculture system was used to quantitate cytolytic T lymphocytes and their precursors (CTL-P), which infiltrate tumors induced by injection of Moloney sarcoma-leukemia virus (MSV-MoLV) complex into C57BL/6 mice. Leukocyte populations obtained from tumors collected on day 10 after virus injection were found to contain significantly higher frequencies of operationally defined (tumor-specific) CTL-P than either peripheral blood leukocytes (PBL) or spleen cells from the same animals. When these frequencies were normalized according to the content of Lyt-2+ T cells in each tissue, average CTL-P frequencies were found to be 1/9 in tumor-infiltrating cells vs. 1/41 in PBL. These results directly demonstrate selective accumulation of CTL-P in the tumor mass. A number of clonal isolates obtained from tumor-infiltrating leukocyte populations were expanded and studied for cytolytic activity and specificity. Of 11 isolates, 10 were found to have high cytolytic activity, leading to 50% lysis of the syngeneic MoLV-derived tumor target cells in 3.5 h at lymphocyte:target cell ratios ranging from 0.5:1 to 3.2:1. Furthermore, five randomly selected clones showed H-2 restriction by their selective lytic activity against MoLV-derived syngeneic MBL-2 target cells and their lack of activity against either MoLV-derived allogeneic (LSTRA) tumor cells or against syngeneic (EL4) or allogeneic (P815) target cells unrelated to MoLV.
Mots-clé
Animals Clone Cells/immunology Cytotoxicity, Immunologic Leukemia, Experimental/*immunology Mice Mice, Inbred C57BL/immunology Moloney murine leukemia virus/immunology Spleen/immunology T-Lymphocytes/*immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
09/05/2019 3:38
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