cis- and trans-acting elements involved in reactivation of vaccinia virus early transcription.

Détails

ID Serval
serval:BIB_F75C745BB743
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
cis- and trans-acting elements involved in reactivation of vaccinia virus early transcription.
Périodique
Journal of virology
Auteur(s)
Masternak K., Wittek R.
ISSN
0022-538X
Statut éditorial
Publié
Date de publication
1996
Peer-reviewed
Oui
Volume
70
Numéro
12
Pages
8737-46
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
We have previously shown that transcription from the vaccinia virus 7.5K early promoter is reactivated late in infection (J. Garcés, K. Masternak, B. Kunz, and R. Wittek, J. Virol. 67:5394-5401, 1993). To identify the sequence elements mediating reactivation, we constructed recombinant viruses harboring deletions, substitutions, or insertions in the 7.5K promoter or its flanking regions. The analysis of these viruses showed that sequences both upstream as well as downstream of the transcription initiation site contribute to reactivation of the 7.5K promoter. We tested whether reactivation could be explained by a high affinity of vaccinia virus early transcription factor to reactivated promoters. Bandshift experiments using purified protein showed that promoters which bind the factor with high affinity in general also have high early transcriptional activity. However, no correlation was found between affinity of the factor and reactivation. Interestingly, overexpression of recombinant early transcription factor in vaccinia virus-infected cells resulted in a shutdown of late transcription and in reactivation of promoters, which are normally not reactivated.
Mots-clé
Animals, Cell Line, Cercopithecus aethiops, DNA Mutational Analysis, Gene Expression Regulation, Viral, Hela Cells, Humans, Mutagenesis, Regulatory Sequences, Nucleic Acid, Transcription Factors, Transcription, Genetic, Vaccinia virus, Viral Proteins
Pubmed
Web of science
Création de la notice
24/01/2008 10:43
Dernière modification de la notice
20/08/2019 16:23
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