Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression
Details
Serval ID
serval:BIB_F74E14052B12
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression
Journal
Molecular and Cellular Biology
ISSN
0270-7306 (Print)
Publication state
Published
Issued date
11/1994
Volume
14
Number
11
Pages
7276-84
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov
Abstract
Hepatocyte nuclear factor 4 (HNF-4) is a liver-enriched transcription factor and a member of the steroid hormone receptor superfamily. HNF-4 is required for the hepatoma-specific expression of HNF-1 alpha, another liver-enriched transcription factor, suggesting the early participation of HNF-4 in development. To prepare for further study of HNF-4 in development, the tissue-specific expression of the mouse HNF-4 gene was studied by analyzing the promoter region for required DNA elements. DNase-hypersensitive sites in the gene in liver and kidney tissues were found in regions both distal and proximal to the RNA start that were absent in tissues in which HNF-4 expression did not occur. By use of reporter constructs in transient-transfection assays and with transgenic mice, a region sufficient to drive liver-specific expression of HNF-4 was identified. While an HNF-1 binding site between bp -98 and -68 played an important role in the hepatoma-specific promoter activity of HNF-4 in transient-transfection assays, it was not sufficient for the liver-specific expression of a reporter gene in transgenic mice. Distal enhancer elements indicated by the presence of DNase I-hypersensitive sites at kb -5.5 and -6.5, while not functional in transient-transfection assays, were required for the correct expression of the mouse HNF-4 gene in animals.
Keywords
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
DNA Probes/genetics
*DNA-Binding Proteins
Deoxyribonuclease I
Gene Expression Regulation
Hepatocyte Nuclear Factor 4
Kidney/metabolism
Liver/metabolism
Liver Neoplasms, Experimental/genetics/metabolism
Mice
Mice, Transgenic
Molecular Sequence Data
*Phosphoproteins
Promoter Regions (Genetics)
RNA/genetics
Tissue Distribution
Transcription Factors/*genetics/metabolism
Transfection
Tumor Cells, Cultured/metabolism
Pubmed
Web of science
Create date
25/01/2008 9:41
Last modification date
20/08/2019 17:23