Article: article from journal or magazin.
Novel use of a whole cell E. coli bioreporter as a urinary exposure biomarker.
Environmental Science and Technology
Bacterial bioreporters have substantial potential for contaminant assessment but their real world application is currently impaired by a lack of sensitivity. Here, we exploit the bioconcentration of chemicals in the urine of animals to facilitate pollutant detection. The shore crab Carcinus maenas was exposed to the organic contaminant 2-hydroxybiphenyl, and urine was screened using an Escherichia coli-based luciferase gene (luxAB) reporter assay specific to this compound. Bioassay measurements differentiated between the original contaminant and its metabolites, quantifying bioconcentration factors of up to one hundred-fold in crab urine. Our results reveal the substantial potential of using bacterial bioreporter assays in real-time monitoring of biological matricesto determine exposure histories, with wide ranging potential for the in situ measurement of xenobiotics in risk assessments and epidemiology.
Animals, Biological Assay/methods, Biological Markers/analysis, Biphenyl Compounds/chemistry, Biphenyl Compounds/urine, Brachyura/metabolism, Calibration, Chromatography, High Pressure Liquid, Chromatography, Liquid, Escherichia coli/cytology, Escherichia coli/metabolism, Genes, Reporter, Mass Spectrometry, Urine/chemistry
Web of science
Last modification date