A chemical proteomics approach to phosphatidylinositol 3-kinase signaling in macrophages.

Détails

ID Serval
serval:BIB_F655FC2DEA91
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A chemical proteomics approach to phosphatidylinositol 3-kinase signaling in macrophages.
Périodique
Molecular and Cellular Proteomics
Auteur(s)
Pasquali C., Bertschy-Meier D., Chabert C., Curchod M.L., Arod C., Booth R., Mechtler K., Vilbois F., Xenarios I., Ferguson C.G., Prestwich G.D., Camps M., Rommel C.
ISSN
1535-9476 (Print)
ISSN-L
1535-9476
Statut éditorial
Publié
Date de publication
2007
Volume
6
Numéro
11
Pages
1829-1841
Langue
anglais
Résumé
Prior work using lipid-based affinity matrices has been done to investigate distinct sets of lipid-binding proteins, and one series of experiments has proven successful in mammalian cells for the proteome-wide identification of lipid-binding proteins. However, most lipid-based proteomics screens require scaled up sample preparation, are often composed of multiple cell types, and are not adapted for simultaneous signal transduction studies. Herein we provide a chemical proteomics strategy that uses cleavable lipid "baits" with broad applicability to diverse biological samples. The novel baits were designed to avoid preparative steps to allow functional proteomics studies when the biological source is a limiting factor. Validation of the chemical baits was first confirmed by the selective isolation of several known endogenous phosphatidylinositol 3-kinase signaling proteins using primary bone marrow-derived macrophages. The use of this technique for cellular proteomics and MS/MS analysis was then demonstrated by the identification of known and potential novel lipid-binding proteins that was confirmed in vitro for several proteins by direct lipid-protein interactions. Further to the identification, the method is also compatible with subsequent signal transduction studies, notably for protein kinase profiling of the isolated lipid-bound protein complexes. Taken together, this integration of minimal scale proteomics, lipid chemistry, and activity-based readouts provides a significant advancement in the ability to identify and study the lipid proteome of single, relevant cell types.
Mots-clé
Animals, Cells, Cultured, Chromatography, Affinity, Intracellular Signaling Peptides and Proteins/chemistry, Intracellular Signaling Peptides and Proteins/isolation & purification, Lipids/chemistry, Macrophages/enzymology, Mice, Phosphatidylinositol 3-Kinases/metabolism, Phosphatidylinositols/chemistry, Proteomics/methods, Signal Transduction
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/10/2012 8:21
Dernière modification de la notice
20/08/2019 16:22
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