lacZ transgenic mice to monitor gene expression in embryo and adult

Détails

ID Serval
serval:BIB_F60ABBEE96D6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
lacZ transgenic mice to monitor gene expression in embryo and adult
Périodique
Brain Research Brain Research Protocols
Auteur(s)
Schmidt A., Tief K., Foletti A., Hunziker A., Penna D., Hummler E., Beermann F.
ISSN
1385-299X (Print)
Statut éditorial
Publié
Date de publication
09/1998
Volume
3
Numéro
1
Pages
54-60
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
In transgenic experiments, lacZ can be used as a reporter gene for activity of a given promoter. Its main advantage is the ease of visualization in situ, on sections or in whole mount preparations, and the availability of simple protocols. In the following, we describe our procedure for detecting promoter activity in transgenic mice, including choice of lacZ vectors, generation of the transgenic mice, and analysis of expression. We had recently used this protocol to detect tyrosinase gene promoter activity in embryonic and adult brain.
Mots-clé
Animals Embryo/physiology Gene Expression Regulation, Developmental/*physiology *Lac Operon Mice Mice, Transgenic *Promoter Regions (Genetics) Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
24/01/2008 13:42
Dernière modification de la notice
20/08/2019 17:22
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