Rab3D is critical for secretory granule maturation in PC12 cells.

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Version: author
Serval ID
serval:BIB_F59F60EFBDAD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rab3D is critical for secretory granule maturation in PC12 cells.
Journal
PloS One
Author(s)
Kögel T., Rudolf R., Hodneland E., Copier J., Regazzi R., Tooze S.A., Gerdes H.H.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
8
Number
3
Pages
e57321
Language
english
Abstract
Neuropeptide- and hormone-containing secretory granules (SGs) are synthesized at the trans-Golgi network (TGN) as immature secretory granules (ISGs) and complete their maturation in the F-actin-rich cell cortex. This maturation process is characterized by acidification-dependent processing of cargo proteins, condensation of the SG matrix and removal of membrane and proteins not destined to mature secretory granules (MSGs). Here we addressed a potential role of Rab3 isoforms in these maturation steps by expressing their nucleotide-binding deficient mutants in PC12 cells. Our data show that the presence of Rab3D(N135I) decreases the restriction of maturing SGs to the F-actin-rich cell cortex, blocks the removal of the endoprotease furin from SGs and impedes the processing of the luminal SG protein secretogranin II. This strongly suggests that Rab3D is implicated in the subcellular localization and maturation of ISGs.
Pubmed
Web of science
Open Access
Yes
Create date
23/07/2013 13:49
Last modification date
20/08/2019 16:22
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