Article: article from journal or magazin.
Immunization of hu-PBL-SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan 16
Antibodies are usually prepared from recently boosted animals and reflect ongoing immune responses. In humans, this is restrictive as ethical constraints generally prevent antigen-boosting. Therefore the rich memory compartment of human antibody responses remains largely untapped. Severe combined immune deficiency (SCID) mice populated with human cells allow the stimulation of human antibody memory without the usual constraints. Here we show how peripheral blood lymphocytes can be stimulated by antigen to produce large secondary responses after transfer to SCID mice. Specific monoclonal human Fab fragments can then be isolated from the mice by repertoire cloning even when the human donor's last contact with antigen was more than 17 years ago.
Amino Acid Sequence Animals Antibodies, Monoclonal/*immunology Base Sequence Gene Library Hepatitis B Core Antigens/immunology Humans Immunization Immunoglobulin Fab Fragments/*immunology Immunoglobulin G/immunology Immunoglobulin Heavy Chains/immunology Immunoglobulin Light Chains *Immunologic Memory Lymphocyte Transfusion Lymphocytes/*immunology Mice Mice, SCID Molecular Sequence Data Oligodeoxyribonucleotides Transplantation, Heterologous
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