Ultra high performance supercritical fluid chromatography coupled with tandem mass spectrometry for screening of doping agents. I: Investigation of mobile phase and MS conditions.

Détails

ID Serval
serval:BIB_F497E1FB507F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ultra high performance supercritical fluid chromatography coupled with tandem mass spectrometry for screening of doping agents. I: Investigation of mobile phase and MS conditions.
Périodique
Analytica Chimica Acta
Auteur(s)
Nováková L., Grand-Guillaume Perrenoud A., Nicoli R., Saugy M., Veuthey J.L., Guillarme D.
ISSN
1873-4324 (Electronic)
ISSN-L
0003-2670
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
853
Pages
637-646
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
The conditions for the analysis of selected doping substances by UHPSFC-MS/MS were optimized to ensure suitable peak shapes and maximized MS responses. A representative mixture of 31 acidic and basic doping agents was analyzed, in both ESI+ and ESI- modes. The best compromise for all compounds in terms of MS sensitivity and chromatographic performance was obtained when adding 2% water and 10mM ammonium formate in the CO2/MeOH mobile phase. Beside mobile phase, the nature of the make-up solvent added for interfacing UHPSFC with MS was also evaluated. Ethanol was found to be the best candidate as it was able to compensate for the negative effect of 2% water addition in ESI- mode and provided a suitable MS response for all doping agents. Sensitivity of the optimized UHPSFC-MS/MS method was finally assessed and compared to the results obtained in conventional UHPLC-MS/MS. Sensitivity was improved by 5-100-fold in UHPSFC-MS/MS vs. UHPLC-MS/MS for 56% of compounds, while only one compound (bumetanide) offered a significantly higher MS response (4-fold) under UHPLC-MS/MS conditions. In the second paper of this series, the optimal conditions for UHPSFC-MS/MS analysis will be employed to screen >100 doping agents in urine matrix and results will be compared to those obtained by conventional UHPLC-MS/MS.
Pubmed
Web of science
Création de la notice
28/01/2015 12:35
Dernière modification de la notice
03/03/2018 22:44
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