Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants.

Détails

ID Serval
serval:BIB_F47BED3DBE36
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants.
Périodique
Nature plants
Auteur(s)
Lorenzo-Orts L., Witthoeft J., Deforges J., Martinez J., Loubéry S., Placzek A., Poirier Y., Hothorn L.A., Jaillais Y., Hothorn M.
ISSN
2055-0278 (Electronic)
ISSN-L
2055-0278
Statut éditorial
Publié
Date de publication
02/2019
Peer-reviewed
Oui
Volume
5
Numéro
2
Pages
184-193
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF). Here, we report the characterization of a distinct bicistronic transcript in Arabidopsis. We analysed loss-of-function phenotypes of the inorganic polyphosphatase TRIPHOSPHATE TUNNEL METALLOENZYME 3 (AtTTM3), and found that catalytically inactive versions of the enzyme could fully complement embryo and growth-related phenotypes. We could rationalize these puzzling findings by characterizing a uORF in the AtTTM3 locus encoding CELL DIVISION CYCLE PROTEIN 26 (CDC26), an orthologue of the cell cycle regulator. We demonstrate that AtCDC26 is part of the plant anaphase promoting complex/cyclosome (APC/C), regulates accumulation of APC/C target proteins and controls cell division, growth and embryo development. AtCDC26 and AtTTM3 are translated from a single transcript conserved across the plant lineage. While there is no apparent biochemical connection between the two gene products, AtTTM3 coordinates AtCDC26 translation by recruiting the transcript into polysomes. Our work highlights that uORFs may encode functional proteins in plant genomes.
Mots-clé
5' Untranslated Regions, Acid Anhydride Hydrolases/genetics, Acid Anhydride Hydrolases/metabolism, Anaphase-Promoting Complex-Cyclosome/genetics, Anaphase-Promoting Complex-Cyclosome/metabolism, Arabidopsis/cytology, Arabidopsis/genetics, Arabidopsis/growth & development, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, CRISPR-Cas Systems, Cytoplasm/metabolism, Gene Expression Regulation, Plant, Mutation, Open Reading Frames, Plants, Genetically Modified, Polyribosomes/genetics, Polyribosomes/metabolism
Pubmed
Web of science
Création de la notice
25/03/2019 10:21
Dernière modification de la notice
20/08/2019 16:21
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