Article: article from journal or magazin.
CD4+CD3- cells induce Peyer's patch development: role of alpha4beta1 integrin activation by CXCR5.
CD4+CD3- cells are the predominant hematopoietic cells found in mouse fetal intestine. We prove their role as Peyer's patch (PP)-inducing cells by transfer into neonatal PP-deficient mice. To test the requirement of chemokines and adhesion molecules in induction of PP, we studied mice deficient in CXCR5 and/or alpha4beta1 integrin-mediated adhesion. CXCR5-/- mice have CD4+CD3- cells, which are inefficient in inducing PP formation. We show here that CXCR5/CXCL13 signaling activates alpha4beta1 integrin on CD4+CD3- cells. Blocking of beta1 integrin or VCAM-1, the ligand of alpha4beta1 integrin, inhibits PP formation. This study demonstrates the link between chemokine receptors and adhesion molecules that regulates stromal/hematopoietic cell interaction leading to PP formation.
Adoptive Transfer, Animals, Antigens, CD3/analysis, CD4-Positive T-Lymphocytes/physiology, CD4-Positive T-Lymphocytes/transplantation, Cell Adhesion, Chemokine CXCL13, Chemokines, CXC/physiology, Immunologic Deficiency Syndromes/genetics, Immunologic Deficiency Syndromes/immunology, Integrin alpha4beta1/physiology, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Immunological, Morphogenesis, Peyer's Patches/growth &, development, Radiation Chimera, Receptors, CXCR5, Receptors, Chemokine, Receptors, Cytokine/deficiency, Receptors, Cytokine/genetics, Signal Transduction/immunology, Stromal Cells/cytology, Vascular Cell Adhesion Molecule-1/physiology
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