Excision and transposition of Tn5 upon insertion in the hha gene of Escherichia coli
Details
Serval ID
serval:BIB_F3AF353471F8
Type
Article: article from journal or magazin.
Collection
Publications
Fund
Title
Excision and transposition of Tn5 upon insertion in the hha gene of Escherichia coli
Journal
Canadian Journal of Microbiology
ISSN
0008-4166 (Print)
Publication state
Published
Issued date
07/1994
Volume
40
Number
7
Pages
597-601
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
We report the occurrence of Tn5 secondary transpositions in Escherichia coli HB101 hha::Tn5(pANN202-312). Tn5-induced hha mutants (hemolysin overproducers) segregated at high frequency either hemolysin-negative clones or clones showing the parental hemolytic phenotype (reduced hemolysin production). Secondary transpositions of Tn5 appeared to be responsible for the phenotypes of both types of derivatives. The hemolysin-negative clones no longer harboured Tn5 in the hha gene, but rather Tn5 was in the hly genes of the hemolytic plasmid pANN202-312. The derivatives that recovered the parental hemolytic phenotype contained a single copy of Tn5 in a chromosomal location different from that of hha. Both kinds of transpositional events appeared to restore the function of the hha gene.
Keywords
Base Sequence Blotting, Southern DNA Probes *DNA Transposable Elements Escherichia coli/*genetics *Genes, Bacterial Hemolysin Proteins/biosynthesis/genetics Molecular Sequence Data Mutation
DOI
Pubmed
Web of science
Create date
28/01/2008 11:55
Last modification date
03/03/2018 21:42