Excision and transposition of Tn5 upon insertion in the hha gene of Escherichia coli

Détails

ID Serval
serval:BIB_F3AF353471F8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Excision and transposition of Tn5 upon insertion in the hha gene of Escherichia coli
Périodique
Canadian Journal of Microbiology
Auteur(s)
Badenas  C., Madrid  C., Juarez  A.
ISSN
0008-4166 (Print)
Statut éditorial
Publié
Date de publication
07/1994
Volume
40
Numéro
7
Pages
597-601
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
We report the occurrence of Tn5 secondary transpositions in Escherichia coli HB101 hha::Tn5(pANN202-312). Tn5-induced hha mutants (hemolysin overproducers) segregated at high frequency either hemolysin-negative clones or clones showing the parental hemolytic phenotype (reduced hemolysin production). Secondary transpositions of Tn5 appeared to be responsible for the phenotypes of both types of derivatives. The hemolysin-negative clones no longer harboured Tn5 in the hha gene, but rather Tn5 was in the hly genes of the hemolytic plasmid pANN202-312. The derivatives that recovered the parental hemolytic phenotype contained a single copy of Tn5 in a chromosomal location different from that of hha. Both kinds of transpositional events appeared to restore the function of the hha gene.
Mots-clé
Base Sequence Blotting, Southern DNA Probes *DNA Transposable Elements Escherichia coli/*genetics *Genes, Bacterial Hemolysin Proteins/biosynthesis/genetics Molecular Sequence Data Mutation
Pubmed
Web of science
Création de la notice
28/01/2008 12:55
Dernière modification de la notice
03/03/2018 22:42
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