Involvement of glucocorticoid prereceptor metabolism and signaling in rat visceral adipose tissue lipid metabolism after chronic stress combined with high-fructose diet.

Details

Serval ID
serval:BIB_F3A7FC8CD251
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Involvement of glucocorticoid prereceptor metabolism and signaling in rat visceral adipose tissue lipid metabolism after chronic stress combined with high-fructose diet.
Journal
Molecular and cellular endocrinology
Author(s)
Bursać B., Djordjevic A., Veličković N., Milutinović D.V., Petrović S., Teofilović A., Gligorovska L., Preitner F., Tappy L., Matić G.
ISSN
1872-8057 (Electronic)
ISSN-L
0303-7207
Publication state
Published
Issued date
15/11/2018
Peer-reviewed
Oui
Volume
476
Pages
110-118
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Both fructose overconsumption and increased glucocorticoids secondary to chronic stress may contribute to overall dyslipidemia. In this study we specifically assessed the effects and interactions of dietary fructose and chronic stress on lipid metabolism in the visceral adipose tissue (VAT) of male Wistar rats. We analyzed the effects of 9-week 20% high fructose diet and 4-week chronic unpredictable stress, separately and in combination, on VAT histology, glucocorticoid prereceptor metabolism, glucocorticoid receptor subcellular redistribution and expression of major metabolic genes. Blood triglycerides and fatty acid composition were also measured to assess hepatic Δ9 desaturase activity. The results showed that fructose diet increased blood triglycerides and Δ9 desaturase activity. On the other hand, stress led to corticosterone elevation, glucocorticoid receptor activation and decrease in adipocyte size, while phosphoenolpyruvate carboxykinase, adipose tissue triglyceride lipase, FAT/CD36 and sterol regulatory element binding protein-1c (SREBP-1c) were increased, pointing to VAT lipolysis and glyceroneogenesis. The combination of stress and fructose diet was associated with marked stimulation of fatty acid synthase and acetyl-CoA carboxylase mRNA level and with increased 11β-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase protein levels, suggesting a coordinated increase in hexose monophosphate shunt and de novo lipogenesis. It however did not influence the level of peroxisome proliferator-activated receptor-gamma, SREBP-1c and carbohydrate responsive element-binding protein. In conclusion, our results showed that only combination of dietary fructose and stress increase glucocorticoid prereceptor metabolism and stimulates lipogenic enzyme expression suggesting that interaction between stress and fructose may be instrumental in promoting VAT expansion and dysfunction.
Keywords
Animals, Corticosterone/blood, Diet, Fatty Acids/blood, Fructose, Gene Expression Regulation, Insulin/blood, Intra-Abdominal Fat/metabolism, Lipid Metabolism/genetics, Male, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rats, Wistar, Receptors, Glucocorticoid/metabolism, Signal Transduction, Stearoyl-CoA Desaturase/metabolism, Stress, Psychological/blood, Stress, Psychological/metabolism, Transcription, Genetic, Triglycerides/blood, Chronic unpredictable stress, Glucocorticoids, Lipid metabolism, Visceral adipose tissue
Pubmed
Web of science
Create date
12/05/2018 9:31
Last modification date
20/08/2019 16:20
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