Molecular characterization of a human matrix attachment region epigenetic regulator.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_F39C96561823
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular characterization of a human matrix attachment region epigenetic regulator.
Périodique
PLoS One
Auteur(s)
Arope S., Harraghy N., Pjanic M., Mermod N.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2013
Volume
8
Numéro
11
Pages
e79262
Langue
anglais
Résumé
Matrix attachment regions (MAR) generally act as epigenetic regulatory sequences that increase gene expression, and they were proposed to partition chromosomes into loop-forming domains. However, their molecular mode of action remains poorly understood. Here, we assessed the possible contribution of the AT-rich core and adjacent transcription factor binding motifs to the transcription augmenting and anti-silencing effects of human MAR 1-68. Either flanking sequences together with the AT-rich core were required to obtain the full MAR effects. Shortened MAR derivatives retaining full MAR activity were constructed from combinations of the AT-rich sequence and multimerized transcription factor binding motifs, implying that both transcription factors and the AT-rich microsatellite sequence are required to mediate the MAR effect. Genomic analysis indicated that MAR AT-rich cores may be depleted of histones and enriched in RNA polymerase II, providing a molecular interpretation of their chromatin domain insulator and transcriptional augmentation activities.
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2014 12:36
Dernière modification de la notice
20/08/2019 16:20
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