Context-dependent dual role of SKI8 homologs in mRNA synthesis and turnover.

Détails

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_F39277485DBF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Context-dependent dual role of SKI8 homologs in mRNA synthesis and turnover.
Périodique
PLoS Genetics
Auteur(s)
Dorcey E., Rodriguez-Villalon A., Salinas P., Santuari L., Pradervand S., Harshman K., Hardtke C.S.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Statut éditorial
Publié
Date de publication
2012
Volume
8
Numéro
4
Pages
e1002652
Langue
anglais
Résumé
Eukaryotic mRNA transcription and turnover is controlled by an enzymatic machinery that includes RNA polymerase II and the 3' to 5' exosome. The activity of these protein complexes is modulated by additional factors, such as the nuclear RNA polymerase II-associated factor 1 (Paf1c) and the cytoplasmic Superkiller (SKI) complex, respectively. Their components are conserved across uni- as well as multi-cellular organisms, including yeast, Arabidopsis, and humans. Among them, SKI8 displays multiple facets on top of its cytoplasmic role in the SKI complex. For instance, nuclear yeast ScSKI8 has an additional function in meiotic recombination, whereas nuclear human hSKI8 (unlike ScSKI8) associates with Paf1c. The Arabidopsis SKI8 homolog VERNALIZATION INDEPENDENT 3 (VIP3) has been found in Paf1c as well; however, whether it also has a role in the SKI complex remains obscure so far. We found that transgenic VIP3-GFP, which complements a novel vip3 mutant allele, localizes to both nucleus and cytoplasm. Consistently, biochemical analyses suggest that VIP3-GFP associates with the SKI complex. A role of VIP3 in the turnover of nuclear encoded mRNAs is supported by random-primed RNA sequencing of wild-type and vip3 seedlings, which indicates mRNA stabilization in vip3. Another SKI subunit homolog mutant, ski2, displays a dwarf phenotype similar to vip3. However, unlike vip3, it displays neither early flowering nor flower development phenotypes, suggesting that the latter reflect VIP3's role in Paf1c. Surprisingly then, transgenic ScSKI8 rescued all aspects of the vip3 phenotype, suggesting that the dual role of SKI8 depends on species-specific cellular context.
Mots-clé
Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Flowers/genetics, Flowers/growth & development, High-Throughput Nucleotide Sequencing, Humans, Meiosis/genetics, Mutation, Nuclear Proteins/genetics, Phenotype, Plants, Genetically Modified, RNA Polymerase II/genetics, RNA Polymerase II/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Species Specificity
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/06/2012 15:14
Dernière modification de la notice
09/05/2019 3:26
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