High-affinity binding of 125I-labelled mouse interferon to a specific cell surface receptor.

Détails

ID Serval
serval:BIB_F37E5C9FCD87
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High-affinity binding of 125I-labelled mouse interferon to a specific cell surface receptor.
Périodique
Nature
Auteur(s)
Aguet M.
ISSN
0028-0836 (Print)
ISSN-L
0028-0836
Statut éditorial
Publié
Date de publication
1980
Volume
284
Numéro
5755
Pages
459-461
Langue
anglais
Résumé
Previous research suggests that interferon binds to the cell surface, possibly by attachment to gangliosides. A two-component receptor system consisting of binding and activation sites has been proposed. This hypothesis is supported by the finding that interferon inhibits binding of cholera toxin and thyrotropin to their receptors suggesting possible common receptor sites. Moreover, an antiserum against cell surface components of interferon-sensitive cells has been shown to inhibit the action of interferon. However, to understand the interaction of interferon with the cell surface requires direct ligand-binding studies. I present here direct evidence that high-affinity binding of interferon to a specific cell surface receptor is an initial step in interferon action using biologically active purified 125I-labelled mouse interferon. Labelled interferon binds specifically to interferon-sensitive mouse leukaemia L1210 cells, whereas binding to interferon-resistant L1210 cells is nonspecific. Furthermore, specific binding to monolayer cultures of mouse L929 cells is compared with nonspecific binding to chick embryo fibroblasts insensitive to the action of mouse interferon.
Mots-clé
Animals, Cell Membrane/metabolism, Chick Embryo, Interferons/metabolism, Kinetics, L Cells (Cell Line), Leukemia L1210/metabolism, Mice, Receptors, Drug/metabolism, Receptors, Interferon, Species Specificity
Pubmed
Web of science
Création de la notice
28/01/2008 12:37
Dernière modification de la notice
03/03/2018 22:42
Données d'usage