Article: article from journal or magazin.
Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice
Biochimica et Biophysica Acta-Gene Structure and Expression
Journal Article Research Support, Non-U.S. Gov't --- Old month value: May 28
We have isolated and characterised the promoter of the mouse Scnn1a (alpha ENaC) gene. Using transient transfections of serial deletion mutants into Scnn1a-expressing cells, we demonstrate that 1.56 kb of 5' upstream sequence is required for cell-specific expression and corticosteroid-mediated regulation. These 5' sequences are not sufficient to drive expression of a lacZ reporter gene or a rat Scnn1a cDNA in transgenic mice, where they failed to rescue Scnn1a deficiency.
Amino Acid Sequence Animals Base Sequence Cells, Cultured Consensus Sequence Epithelial Sodium Channel Gene Expression Regulation Kidney Cortex/*metabolism Kidney Tubules, Collecting/*metabolism Mice Mice, Transgenic Molecular Sequence Data *Promoter Regions (Genetics) Sodium Channels/*genetics Transfection
Web of science
Last modification date