Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice

Détails

ID Serval
serval:BIB_F35C8F3D41CC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice
Périodique
Biochimica et Biophysica Acta-Gene Structure and Expression
Auteur(s)
Kohler S., Pradervand S., Verdumo C., Mérillat A. M., Bens M., Vandewalle A., Beermann F., Hummler E.
ISSN
0167-4781
ISSN-L
1879-2634
Statut éditorial
Publié
Date de publication
05/2001
Peer-reviewed
Oui
Volume
1519
Numéro
1-2
Pages
106-10
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: May 28
Résumé
We have isolated and characterised the promoter of the mouse Scnn1a (alpha ENaC) gene. Using transient transfections of serial deletion mutants into Scnn1a-expressing cells, we demonstrate that 1.56 kb of 5' upstream sequence is required for cell-specific expression and corticosteroid-mediated regulation. These 5' sequences are not sufficient to drive expression of a lacZ reporter gene or a rat Scnn1a cDNA in transgenic mice, where they failed to rescue Scnn1a deficiency.
Mots-clé
Amino Acid Sequence Animals Base Sequence Cells, Cultured Consensus Sequence Epithelial Sodium Channel Gene Expression Regulation Kidney Cortex/*metabolism Kidney Tubules, Collecting/*metabolism Mice Mice, Transgenic Molecular Sequence Data *Promoter Regions (Genetics) Sodium Channels/*genetics Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 13:42
Dernière modification de la notice
20/08/2019 17:20
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