Micromeasurement of total and regional CO2 productions in the one-day-old chick embryo.

Détails

ID Serval
serval:BIB_F2B8E99EF70B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Micromeasurement of total and regional CO2 productions in the one-day-old chick embryo.
Périodique
Respiration Physiology
Auteur(s)
Raddatz E., Kucera P., de Ribaupierre Y.
ISSN
0034-5687 (Print)
ISSN-L
0034-5687
Statut éditorial
Publié
Date de publication
1987
Volume
70
Numéro
1
Pages
1-11
Langue
anglais
Résumé
A conductometric micromethod combined with image analysis system has been developed allowing to determine the CO2 production within 'two-dimensional' tissues, i.e., flat and thin cell layers or epithelial sheets. The preparation was mounted into an airtight chamber separated in two compartments by a thin silicone membrane permeable to gases. The lower compartment contained the nutritive medium and the preparation. The upper compartment and a conductivity measuring capillary connected in series were perfused with a solution of Ba(OH)2. The CO2 produced by the tissue precipitated as BaCO3 and the resulting decrease of electrical conductivity was linearly related to the total CO2 production. In addition, the pattern of CO2 production was directly observable as the BaCO3 crystals formed upon the silicone membrane over the regions which produced CO2. The spatial distribution of the crystals was quantified by video image processing and the regional CO2 production evaluated with a spatial resolution of 100 microns. This new microtechnique was originally developed to study the CO2 production in the early chick blastoderm which is a disc 1-5 cells thick. At the stage of young neurula the CO2 production was found to be 235 +/- 37 nmol.h-1 (mean +/- SD; n = 10) per blastoderm and large variations of local CO2 production were detected from one region to another (from 0.6 to 6.5 nmol.h-1.mm-2). These results indicate a high metabolic and functional differentiation of cells within the blastoderm. The possible applications and improvements of such a microtechnique are discussed.
Mots-clé
Animals, Blastoderm/metabolism, Carbon Dioxide/biosynthesis, Chick Embryo, Conductometry/methods
Pubmed
Web of science
Création de la notice
24/01/2008 14:19
Dernière modification de la notice
03/03/2018 22:40
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