Akt signalling through GSK-3beta, mTOR and Foxo1 is involved in human skeletal muscle hypertrophy and atrophy

Détails

ID Serval
serval:BIB_F29566DC12F3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Akt signalling through GSK-3beta, mTOR and Foxo1 is involved in human skeletal muscle hypertrophy and atrophy
Périodique
Journal of Physiology
Auteur(s)
Leger B., Cartoni R., Praz M., Lamon S., Deriaz O., Crettenand A., Gobelet C., Rohmer P., Konzelmann M., Luthi F., Russell A. P.
ISSN
0022-3751
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
576
Numéro
Pt 3
Pages
923-933
Langue
anglais
Résumé
Skeletal muscle size is tightly regulated by the synergy between anabolic and catabolic signalling pathways which, in humans, have not been well characterized. Akt has been suggested to play a pivotal role in the regulation of skeletal muscle hypertrophy and atrophy in rodents and cells. Here we measured the amount of phospho-Akt and several of its downstream anabolic targets (glycogen synthase kinase-3beta (GSK-3beta), mTOR, p70(s6k) and 4E-BP1) and catabolic targets (Foxo1, Foxo3, atrogin-1 and MuRF1). All measurements were performed in human quadriceps muscle biopsies taken after 8 weeks of both hypertrophy-stimulating resistance training and atrophy-stimulating de-training. Following resistance training a muscle hypertrophy ( approximately 10%) and an increase in phospho-Akt, phospho-GSK-3beta and phospho-mTOR protein content were observed. This was paralleled by a decrease in Foxo1 nuclear protein content. Following the de-training period a muscle atrophy (5%), relative to the post-training muscle size, a decrease in phospho-Akt and GSK-3beta and an increase in Foxo1 were observed. Atrogin-1 and MuRF1 increased after the hypertrophy and decreased after the atrophy phases. We demonstrate, for the first time in human skeletal muscle, that the regulation of Akt and its downstream signalling pathways GSK-3beta, mTOR and Foxo1 are associated with both the skeletal muscle hypertrophy and atrophy processes
Mots-clé
Adaptor Proteins,Signal Transducing , Adult , Atrophy , Exercise , Forkhead Transcription Factors , Gene Expression Regulation , genetics , Glycogen Synthase Kinase 3 , Humans , Hypertrophy , Male , metabolism , Muscle Proteins , Muscle,Skeletal , pathology , Phosphoproteins , physiology , Protein Kinases , Proto-Oncogene Proteins c-akt , Ribosomal Protein S6 Kinases,70-kDa , RNA,Messenger , Signal Transduction , SKP Cullin F-Box Protein Ligases , Ubiquitin-Protein Ligases
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/11/2008 18:34
Dernière modification de la notice
09/05/2019 3:23
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