Permissivity of fish cell lines to three Chlamydia-related bacteria: Waddlia chondrophila, Estrella lausannensis and Parachlamydia acanthamoebae.

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Serval ID
serval:BIB_F26B714D76C8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Permissivity of fish cell lines to three Chlamydia-related bacteria: Waddlia chondrophila, Estrella lausannensis and Parachlamydia acanthamoebae.
Journal
Fems Immunology and Medical Microbiology
Author(s)
Kebbi-Beghdadi C., Batista C., Greub G.
ISSN
1574-695X (Electronic)
ISSN-L
0928-8244
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
63
Number
3
Pages
339-345
Language
english
Abstract
Epitheliocystis is an infectious disease affecting gills and skin of various freshwater and marine fishes, associated with high mortality and reduced growth of survivors. Candidatus Piscichlamydia salmonis and Clavochlamydia salmonicola have recently been identified as aetiological agents of epitheliocystis in Atlantic Salmon. In addition, several other members of the Chlamydiales order have been identified in other fish species. To clarify the pathogenicity of Chlamydia-like organisms towards fishes, we investigated the permissivity of two fish cell lines, EPC-175 (Fathead Minnow) and RTG-2 (rainbow trout) to three Chlamydia-related bacteria: Waddlia chondrophila, Parachlamydia acanthamoebae and Estrella lausannensis. Quantitative PCR and immunofluorescence demonstrated that W. chondrophila and, to a lesser extent, E. lausannensis were able to replicate in the two cell lines tested. Waddlia chondrophila multiplied rapidly in its host cell and a strong cytopathic effect was observed. During E. lausannensis infection, we observed a limited replication of the bacteria not followed by host cell lysis. Very limited replication of P. acanthamoebae was observed in both cell lines tested. Given its high infectivity and cytopathic effect towards fish cell lines, W. chondrophila represents the most interesting Chlamydia-related bacteria to be used to develop an in vivo model of epitheliocystis disease in fishes.
Keywords
Animals, Cell Death, Cell Line, Chlamydiales/growth & development, Chlamydiales/pathogenicity, DNA, Bacterial/biosynthesis, DNA, Bacterial/genetics, Fishes, Fluorescent Antibody Technique, Host Specificity, Real-Time Polymerase Chain Reaction, Virulence
Pubmed
Web of science
Open Access
Yes
Create date
03/04/2016 21:03
Last modification date
20/08/2019 16:19
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