Permissivity of fish cell lines to three Chlamydia-related bacteria: Waddlia chondrophila, Estrella lausannensis and Parachlamydia acanthamoebae.

Détails

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_F26B714D76C8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Permissivity of fish cell lines to three Chlamydia-related bacteria: Waddlia chondrophila, Estrella lausannensis and Parachlamydia acanthamoebae.
Périodique
Fems Immunology and Medical Microbiology
Auteur(s)
Kebbi-Beghdadi C., Batista C., Greub G.
ISSN
1574-695X (Electronic)
ISSN-L
0928-8244
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
63
Numéro
3
Pages
339-345
Langue
anglais
Résumé
Epitheliocystis is an infectious disease affecting gills and skin of various freshwater and marine fishes, associated with high mortality and reduced growth of survivors. Candidatus Piscichlamydia salmonis and Clavochlamydia salmonicola have recently been identified as aetiological agents of epitheliocystis in Atlantic Salmon. In addition, several other members of the Chlamydiales order have been identified in other fish species. To clarify the pathogenicity of Chlamydia-like organisms towards fishes, we investigated the permissivity of two fish cell lines, EPC-175 (Fathead Minnow) and RTG-2 (rainbow trout) to three Chlamydia-related bacteria: Waddlia chondrophila, Parachlamydia acanthamoebae and Estrella lausannensis. Quantitative PCR and immunofluorescence demonstrated that W. chondrophila and, to a lesser extent, E. lausannensis were able to replicate in the two cell lines tested. Waddlia chondrophila multiplied rapidly in its host cell and a strong cytopathic effect was observed. During E. lausannensis infection, we observed a limited replication of the bacteria not followed by host cell lysis. Very limited replication of P. acanthamoebae was observed in both cell lines tested. Given its high infectivity and cytopathic effect towards fish cell lines, W. chondrophila represents the most interesting Chlamydia-related bacteria to be used to develop an in vivo model of epitheliocystis disease in fishes.
Mots-clé
Animals, Cell Death, Cell Line, Chlamydiales/growth & development, Chlamydiales/pathogenicity, DNA, Bacterial/biosynthesis, DNA, Bacterial/genetics, Fishes, Fluorescent Antibody Technique, Host Specificity, Real-Time Polymerase Chain Reaction, Virulence
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/04/2016 22:03
Dernière modification de la notice
09/05/2019 3:22
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