Proteomics of methylene blue photo-treated plasma before and after removal of the dye by an absorbent filter.

Détails

ID Serval
serval:BIB_F200347C1F9D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Proteomics of methylene blue photo-treated plasma before and after removal of the dye by an absorbent filter.
Périodique
Proteomics
Auteur(s)
Crettaz D., Sensebe L., Vu D.H., Schneider P., Depasse F., Bienvenut W.V., Quadroni M., Tissot J.D.
ISSN
1615-9853[print], 1615-9853[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
4
Numéro
3
Pages
881-891
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Methylene blue (MB) and light are used for virus inactivation of plasma for transfusion. However, the presence of MB has been the subject of concern, and efforts have been made to efficiently remove the dye after photo-treatment. For this study, plasma was collected by apheresis from 10 donors (group A), then treated using the MacoPharma THERAFLEX procedure (MB; 1 microM, and light exposure; 180 J/cm(2)) (group B), and finally filtered in order to remove the dye (group C). Proteins were analyzed by two-dimensional electrophoresis, and peptides showing modifications were characterized by mass spectrometry. Clottable and antigenic fibrinogen levels, as well as fibrin polymerization time were measured. Analyses of the gels focused on a region corresponding to pI between 4.5 and 6.5, and M(r) from 7000 to 58 000. In this area, 387 +/- 47 spots matched, and four of these spots presented significant modifications. They corresponded to changes of the gamma-chain of fibrinogen, of transthyretin, and of apolipoprotein A-I, respectively. A decrease of clottable fibrinogen and a prolongation of fibrin polymerization time were observed in groups B and C. Removal of MB by filtration was not responsible for additional protein alterations. The effect of over-treatment of plasma by very high concentrations of MB (50 microM) in association with prolonged light exposure (3 h) was also analyzed, and showed complex alterations of most of the plasma proteins, including fibrinogen gamma-chain, transthyretin, and apolipoprotein A-I. Our data indicates that MB treatment at high concentration and prolonged illumination severely injure plasma proteins. By contrast, at the MB concentration used to inactivate viruses, damages are apparently very restricted.
Mots-clé
Amino Acid Sequence, Blood Donors, Chromatography, Liquid, Coloring Agents/pharmacology, Computational Biology, Electrophoresis, Gel, Two-Dimensional/methods, Fibrin/chemistry, Fibrinogen/chemistry, Humans, Hydrogen-Ion Concentration, Light, Mass Spectrometry, Methylene Blue/pharmacology, Molecular Sequence Data, Peptides/chemistry, Sensitivity and Specificity, Silver Staining
Pubmed
Web of science
Création de la notice
24/01/2008 16:46
Dernière modification de la notice
03/03/2018 22:39
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