Methylation signature of lymph node metastases in breast cancer patients.

Détails

Ressource 1Télécharger: BIB_F1EB0B6A944B.P001.pdf (1325.63 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_F1EB0B6A944B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Methylation signature of lymph node metastases in breast cancer patients.
Périodique
BMC Cancer
Auteur(s)
Barekati Z., Radpour R., Lu Q., Bitzer J., Zheng H., Toniolo P., Lenner P., Zhong X.Y.
ISSN
1471-2407 (Electronic)
ISSN-L
1471-2407
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
12
Pages
244 [8 p.]
Langue
anglais
Notes
Publication types: Journal Article
Résumé
BACKGROUND: Invasion and metastasis are two important hallmarks of malignant tumors caused by complex genetic and epigenetic alterations. The present study investigated the contribution of aberrant methylation profiles of cancer related genes, APC, BIN1, BMP6, BRCA1, CST6, ESR-b, GSTP1, P14 (ARF), P16 (CDKN2A), P21 (CDKN1A), PTEN, and TIMP3, in the matched axillary lymph node metastasis in comparison to the primary tumor tissue and the adjacent normal tissue from the same breast cancer patients to identify the potential of candidate genes methylation as metastatic markers.
METHODS: The quantitative methylation analysis was performed using the SEQUENOM's EpiTYPER? assay which relies on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
RESULTS: The quantitative DNA methylation analysis of the candidate genes showed higher methylation proportion in the primary tumor tissue than that of the matched normal tissue and the differences were significant for the APC, BIN1, BMP6, BRCA1, CST6, ESR-b, P16, PTEN and TIMP3 promoter regions (P<0.05). Among those candidate methylated genes, APC, BMP6, BRCA1 and P16 displayed higher methylation proportion in the matched lymph node metastasis than that found in the normal tissue (P<0.05). The pathway analysis revealed that BMP6, BRCA1 and P16 have a role in prevention of neoplasm metastasis.
CONCLUSIONS: The results of the present study showed methylation heterogeneity between primary tumors and metastatic lesion. The contribution of aberrant methylation alterations of BMP6, BRCA1 and P16 genes in lymph node metastasis might provide a further clue to establish useful biomarkers for screening metastasis.
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/10/2012 18:14
Dernière modification de la notice
09/05/2019 3:20
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