Can serum cytokine profile discriminate irritant-induced and allergen-induced symptoms? A cross-sectional study in workers mostly exposed to laboratory animals

Détails

ID Serval
serval:BIB_F1E17A42D149
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Can serum cytokine profile discriminate irritant-induced and allergen-induced symptoms? A cross-sectional study in workers mostly exposed to laboratory animals
Périodique
Occupational and Environmental Medicine
Auteur(s)
Lemaire Muriel, Oppliger Anne, Hotz Philipp, Renauld Jean-Christophe, Braun Julia, Maggi Marion, Barresi Fabio, Schmid-Grendelmeier Peter, Huaux François, Dressel Holger
ISSN
1470-7926 (Electronic)
ISSN-L
1351-0711
Statut éditorial
Publié
Date de publication
08/2017
Peer-reviewed
Oui
Volume
74
Numéro
8
Pages
592-600
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Résumé
In workers exposed mostly to laboratory animals (LA), symptoms may be due to irritants or allergens. Correct aetiological diagnosis is important for health surveillance.
This study aims to test whether work-related (WR) allergen-induced symptoms are associated with a cytokine profile distinct from that due to irritants.
In a cross-sectional study (n=114), WR respiratory and/or skin symptoms were assessed through a standardised clinical examination and sensitisation to rat and/or mouse allergen determined by serum immunoglobulin E. Serum cytokine concentrations were measured by multiplex assays. The predefined cytokine profiles 'sensitiser' (interleukin (IL)-4, IL-5, IL-13, eotaxin-1) and 'irritation' (IL-8, IL-17A, IL-17F, IL-22) were considered positive, when ≥3 concentrations exceeded the 95th percentile of the asymptomatic non-sensitised group. Results were examined by hierarchical clustering analyses (HCA) and multiple linear regression. Explorative analyses were carried out for nine additional cytokines. Exposure to allergens and endotoxin was assessed in a subpopulation.
The prevalence of the profile 'irritation' was comparable in 28 symptomatic non-sensitised workers and 71 asymptomatic non-sensitised workers. HCA showed that nearly all symptomatic non-sensitised workers were gathered in two subclusters, characterised by high IL-17A levels, but different IL-8 levels. Multiple linear regression identified drug consumption and current complaints as confounders. Sensitised subjects were too few (n=14) for testing the profile 'sensitiser'.
In this unselected population of LA workers, the profile 'irritation' did not prove to be a valuable health surveillance tool. Low power precluded assessment of the profile 'sensitiser'. The increased IL-17A concentration may originate from irritative constituents of organic dust.

Mots-clé
Adolescent, Adult, Animals, Animals, Laboratory, Cross-Sectional Studies, Cytokines/blood, Female, Humans, Hypersensitivity/blood, Hypersensitivity/epidemiology, Hypersensitivity/immunology, Immunoglobulin E/blood, Interleukins/blood, Interviews as Topic, Male, Mice/immunology, Middle Aged, Occupational Diseases/epidemiology, Occupational Diseases/immunology, Occupational Exposure/adverse effects, Rats/immunology, Regression Analysis, Spirometry, Universities, Young Adult, allergy, animal technicians, cytokines
Pubmed
Web of science
Création de la notice
15/08/2017 12:42
Dernière modification de la notice
03/03/2018 22:39
Données d'usage