Changes of MAP2 phosphorylation during brain development.

Détails

ID Serval
serval:BIB_F1DAB16514CD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Changes of MAP2 phosphorylation during brain development.
Périodique
Journal of Histochemistry and Cytochemistry
Auteur(s)
Riederer B.M., Draberova E., Viklicky V., Draber P.
ISSN
0022-1554 (Print)
ISSN-L
0022-1554
Statut éditorial
Publié
Date de publication
1995
Volume
43
Numéro
12
Pages
1269-1284
Langue
anglais
Résumé
The microtubule-associated protein MAP2 is essential for development of early neuronal morphology and maintenance of adult neuronal morphology. Several splice variants exist, MAP2a-d, with a lack of MAP2a in cat brain. MAP2 is widely used as a neuronal marker. In this study we compared five monoclonal antibodies (MAbs) against MAP2. They show differences in the immunocytochemical distribution of MAP2 isoforms during development of the visual cortex and cerebellum of the cat. Local and temporal differences were seen with MAb AP18, an antibody directed against a phosphorylation-dependent epitope near the N-terminal end. In large pyramidal dendrites in visual cortex, the AP18 epitope remained in parts immunoreactive after treatment with alkaline phosphatase. Three MAbs, AP14, MT-01, and MT-02, recognized the central region of the MAP2b molecule, which is not present in MAP2c and 2d, and reacted with phosphorylation-independent epitopes. During the first postnatal week the immunostaining in cerebellum differed between antibodies in that some cellular elements in external and internal granular layers and Purkinje cells were stained to various degrees, whereas at later stages staining patterns were similar. At early stages, antibody MT-02 stained cell bodies and dendrites in cerebral cortex and cerebellum. With progressing maturation, immunoreactivity became restricted to distal parts of apical dendrites of pyramidal cells and was absent from perikarya and finer proximal dendrites in cortex. MT-02 did not stain MAP2 in cerebellum of adult animals. This study demonstrates that the immunocytochemical detection of MAP2 depends on modifications such as phosphorylation and conformational changes of the molecule, and that MAP2 staining patterns differ between MAbs. Phosphorylation and specific conformations in the molecule may be essential for modulating function and molecular stability of MAP2, and monoclonal antibodies against such sites may provide tools for studying the functional role of modifications.
Mots-clé
Animals, Blotting, Western, Brain/growth & development, Cats, Epitope Mapping, Immunohistochemistry, Microtubule-Associated Proteins/metabolism, Phosphorylation, Visual Cortex/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:34
Dernière modification de la notice
03/03/2018 22:39
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