T cell recognition of hapten. Anatomy of T cell receptor binding of a H-2kd-associated photoreactive peptide derivative.
Details
Serval ID
serval:BIB_F1D861FDE466
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
T cell recognition of hapten. Anatomy of T cell receptor binding of a H-2kd-associated photoreactive peptide derivative.
Journal
The Journal of biological chemistry
ISSN
0021-9258
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
274
Number
6
Pages
3622-3631
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
To elucidate the structural basis of T cell recognition of hapten-modified antigenic peptides, we studied the interaction of the T1 T cell antigen receptor (TCR) with its ligand, the H-2Kd-bound Plasmodium berghei circumsporozoite peptide 252-260 (SYIPSAEKI) containing photoreactive 4-azidobenzoic acid (ABA) on P. berghei circumsporozoite Lys259. The photoaffinity-labeled TCR residue(s) were mapped as Tyr48 and/or Tyr50 of complementary determining region 2beta (CDR2beta). Other TCR-ligand contacts were identified by mutational analysis. Molecular modeling, based on crystallographic coordinates of closely related TCR and major histocompatibility complex I molecules, indicated that ABA binds strongly and specifically in a cavity between CDR3alpha and CDR2beta. We conclude that TCR expressing selective Vbeta and CDR3alpha sequences form a binding domain between CDR3alpha and CDR2beta that can accommodate nonpeptidic moieties conjugated at the C-terminal portion of peptides binding to major histocompatibility complex (MHC) encoded proteins.
Keywords
Amino Acid Sequence, Cell Line, H-2 Antigens/chemistry, Haptens/immunology, Models, Molecular, Molecular Sequence Data, Mutagenesis, Peptide Mapping, Peptides/chemistry, Peptides/immunology, Photoaffinity Labels, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, T-Lymphocytes/immunology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:19
Last modification date
20/08/2019 17:19