T cell recognition of hapten. Anatomy of T cell receptor binding of a H-2kd-associated photoreactive peptide derivative.

Détails

ID Serval
serval:BIB_F1D861FDE466
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
T cell recognition of hapten. Anatomy of T cell receptor binding of a H-2kd-associated photoreactive peptide derivative.
Périodique
The Journal of biological chemistry
Auteur(s)
Kessler B., Michielin O., Blanchard C.L., Apostolou I., Delarbre C., Gachelin G., Grégoire C., Malissen B., Cerottini J.C., Wurm F., Karplus M., Luescher I.F.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
274
Numéro
6
Pages
3622-3631
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
To elucidate the structural basis of T cell recognition of hapten-modified antigenic peptides, we studied the interaction of the T1 T cell antigen receptor (TCR) with its ligand, the H-2Kd-bound Plasmodium berghei circumsporozoite peptide 252-260 (SYIPSAEKI) containing photoreactive 4-azidobenzoic acid (ABA) on P. berghei circumsporozoite Lys259. The photoaffinity-labeled TCR residue(s) were mapped as Tyr48 and/or Tyr50 of complementary determining region 2beta (CDR2beta). Other TCR-ligand contacts were identified by mutational analysis. Molecular modeling, based on crystallographic coordinates of closely related TCR and major histocompatibility complex I molecules, indicated that ABA binds strongly and specifically in a cavity between CDR3alpha and CDR2beta. We conclude that TCR expressing selective Vbeta and CDR3alpha sequences form a binding domain between CDR3alpha and CDR2beta that can accommodate nonpeptidic moieties conjugated at the C-terminal portion of peptides binding to major histocompatibility complex (MHC) encoded proteins.
Mots-clé
Amino Acid Sequence, Cell Line, H-2 Antigens/chemistry, Haptens/immunology, Models, Molecular, Molecular Sequence Data, Mutagenesis, Peptide Mapping, Peptides/chemistry, Peptides/immunology, Photoaffinity Labels, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, T-Lymphocytes/immunology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:19
Dernière modification de la notice
09/05/2019 3:20
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