Article: article from journal or magazin.
K-ras and p53 mutations in hereditary non-polyposis colorectal cancers
International Journal of Cancer
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Genetic instability related to defective DNA mismatch repair genes may be involved in the pathogenesis of carcinoma in Hereditary Non-Polyposis Colorectal Cancer (HNPCC). To test that the targets of genetic instability could include critical transforming genes involved in colon tumor progression, we examined 23 colorectal carcinomas in patients with HNPCC in order to detect somatic mutations in K-ras and p53 genes. Using single strand conformation polymorphism followed by direct DNA sequencing, we detected 4 mutations in K-ras gene (17%) and 3 in p53 gene (13%) which change the amino acid sequence of the protein p53. This is significantly lower than in sporadic cancer. Our data suggest that colon cancer in HNPCC might partly involve a distinct pathogenetic mechanism that involves other genes than those altered in sporadic tumors
Adult/Aged/Colorectal Neoplasms,Hereditary Nonpolyposis/genetics/mortality/Pathology/DNA Primers/Female/Genes,p53/Genes,ras/Humans/Male/Middle Aged/Mutation/Neoplasm Staging/Polymorphism,Single-Stranded Conformational/Survival Rate/Tumor Suppressor Protein p53/biosynthesis
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