AAV2 vectors mediate efficient and sustained transduction of rat embryonic ventral mesencephalon.

Détails

ID Serval
serval:BIB_F191385246F5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
AAV2 vectors mediate efficient and sustained transduction of rat embryonic ventral mesencephalon.
Périodique
Neuroreport
Auteur(s)
Lehtonen E., Bonnaud F., Melas C., Lubansu A., Malgrange B., Chtarto A., Velu T., Brotchi J., Levivier M., Peschanski M., Tenenbaum L.
ISSN
0959-4965 (Print)
ISSN-L
0959-4965
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
13
Numéro
12
Pages
1503-1507
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The success of transplantation of human embryonic mesencephalic tissue to treat parkinsonian patients is limited by the poor survival of the transplant. We show that an AAV2 vector mediates efficient expression of the egfp reporter gene in organotypic cultures of freshly explanted solid fragments of rat embryonic ventral mesencephalon (VM). We observed early and sustained transgene expression (4 days to > or = 6 weeks). Furthermore, rAAV-infected rat embryonic VM transplanted in the adult striatum continued to express EGFP for > or = 3 months. More than 95% of the transduced cells were neurons. Dopaminergic neurons were transduced at low frequency at earlier time points. This method of gene delivery could prove useful to achieve local, continuous secretion of neurotrophic factors at physiologically relevant doses to treat Parkinson's disease.
Mots-clé
Animals, Brain Tissue Transplantation, Dopamine/physiology, Fetal Tissue Transplantation, Gene Expression/physiology, Genetic Vectors, Graft Survival/physiology, Green Fluorescent Proteins, Indicators and Reagents/metabolism, Luminescent Proteins/genetics, Male, Mesencephalon/transplantation, Nerve Degeneration/physiopathology, Organ Culture Techniques, Parkinsonian Disorders/surgery, Rats, Rats, Wistar
Pubmed
Web of science
Création de la notice
20/01/2008 18:35
Dernière modification de la notice
03/03/2018 22:38
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