Expression of specific tau exons in normal and tumoral pancreatic acinar cells.

Détails

ID Serval
serval:BIB_F18B576367F5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression of specific tau exons in normal and tumoral pancreatic acinar cells.
Périodique
Journal of Cell Science
Auteur(s)
Vanier M.T., Neuville P., Michalik L., Launay J.F.
ISSN
0021-9533[print], 0021-9533[linking]
Statut éditorial
Publié
Date de publication
05/1998
Volume
111 ( Pt 10)
Pages
1419-1432
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Tau is a neuron-specific microtubule-associated protein (MAP) that is required for the development and maintaining neuronal cell polarity. Tau is encoded by a single gene, while its transcript undergoes a complex and regulated alternative splicing. We have recently reported that tau-like MAPs of 48-55 kDa, corresponding to 6 kb mRNA on northern blots, are expressed in pancreatic acinar cells. In the present study, the expression of tau exons in normal and tumoral pancreatic acinar cells was investigated by RT-PCR and cDNA sequencing. Tau isoforms with four tubulin-binding motifs containing either none, one or two N-terminal inserts (exons 2, 3) are indiscriminately expressed in normal and tumoral cells. However, tau transcripts containing the sequence encoded by exon 6 are specifically expressed in pancreatic tumoral cells from exocrine origin. By immunofluorescence and electron microscopy, we have identified in cellular extensions of tumoral cells, tau-decorated microtubules arranged in bundles like those found in neuronal processes. Tau antisense oligonucleotides inhibit the development of these cellular processes and the expression of the 55 kDa tau isoform.
Mots-clé
Amylases/secretion, Animals, Cloning, Molecular, DNA Primers, Exons/physiology, Gene Expression Regulation, Neoplastic, Microtubules/physiology, Molecular Sequence Data, Pancreas/chemistry, Pancreas/cytology, Pancreatic Neoplasms, Polymerase Chain Reaction, Protein Structure, Tertiary, RNA, Messenger/analysis, Rats, Sequence Homology, Amino Acid, Tumor Cells, Cultured/chemistry, Tumor Cells, Cultured/metabolism, tau Proteins/chemistry, tau Proteins/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 16:44
Dernière modification de la notice
03/03/2018 22:38
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