Identification of cancer/testis-antigen genes by massively parallel signature sequencing.

Détails

ID Serval
serval:BIB_F162E516267A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification of cancer/testis-antigen genes by massively parallel signature sequencing.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Chen Y.T., Scanlan M.J., Venditti C.A., Chua R., Theiler G., Stevenson B.J., Iseli C., Gure A.O., Vasicek T., Strausberg R.L., Jongeneel C.V., Old L.J., Simpson A.J.
ISSN
0027-8424[print], 0027-8424[linking]
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
102
Numéro
22
Pages
7940-7945
Langue
anglais
Résumé
Massively parallel signature sequencing (MPSS) generates millions of short sequence tags corresponding to transcripts from a single RNA preparation. Most MPSS tags can be unambiguously assigned to genes, thereby generating a comprehensive expression profile of the tissue of origin. From the comparison of MPSS data from 32 normal human tissues, we identified 1,056 genes that are predominantly expressed in the testis. Further evaluation by using MPSS tags from cancer cell lines and EST data from a wide variety of tumors identified 202 of these genes as candidates for encoding cancer/testis (CT) antigens. Of these genes, the expression in normal tissues was assessed by RT-PCR in a subset of 166 intron-containing genes, and those with confirmed testis-predominant expression were further evaluated for their expression in 21 cancer cell lines. Thus, 20 CT or CT-like genes were identified, with several exhibiting expression in five or more of the cancer cell lines examined. One of these genes is a member of a CT gene family that we designated as CT45. The CT45 family comprises six highly similar (>98% cDNA identity) genes that are clustered in tandem within a 125-kb region on Xq26.3. CT45 was found to be frequently expressed in both cancer cell lines and lung cancer specimens. Thus, MPSS analysis has resulted in a significant extension of our knowledge of CT antigens, leading to the discovery of a distinctive X-linked CT-antigen gene family.
Mots-clé
Antigens, Neoplasm/genetics, Base Sequence, Cell Line, Tumor, Chromosomes, Human, X/genetics, Computational Biology, DNA Primers, DNA, Complementary/genetics, Databases, Nucleic Acid, Expressed Sequence Tags, Gene Expression Profiling/methods, Humans, Male, Molecular Sequence Data, Multigene Family/genetics, RNA, Messenger/genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, RNA/methods, Testis/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:40
Dernière modification de la notice
09/05/2019 3:19
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