CCL21 is sufficient to mediate DC migration, maturation and function in the absence of CCL19.

Détails

ID Serval
serval:BIB_F1410547061C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CCL21 is sufficient to mediate DC migration, maturation and function in the absence of CCL19.
Périodique
European Journal of Immunology
Auteur(s)
Britschgi M.R., Favre S., Luther S.A.
ISSN
1521-4141[electronic], 0014-2980[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
40
Numéro
5
Pages
1266-1271
Langue
anglais
Résumé
Mice deficient in CCR7 signals show severe defects in lymphoid tissue architecture and immune response. These defects are due to impaired attraction of CCR7+ DC and CCR7+ T cells into the T zones of secondary lymphoid organs and altered DC maturation. It is currently unclear which CCR7 ligand mediates these processes in vivo as CCL19 and CCL21 show an overlapping expression pattern and blocking experiments have given contradictory results. In this study, we addressed this question using CCL19-deficient mice expressing various levels of CCL21. Complete deficiency of CCL19 and CCL21 but not CCL19 alone was found to be associated with abnormal frequencies and localization of DC in naïve LN. Similarly, CCL19 was not required for DC migration from the skin, full DC maturation and efficient T-cell priming. Our findings suggest that CCL21 is the critical CCR7 ligand regulating DC homeostasis and function in vivo with CCL19 being redundant for these processes.
Mots-clé
Alleles, Animals, Bone Marrow Cells/cytology, Bone Marrow Cells/immunology, Cell Differentiation/physiology, Cell Movement/physiology, Chemokine CCL19/deficiency, Chemokine CCL19/genetics, Chemokine CCL21/biosynthesis, Chemokine CCL21/deficiency, Dendritic Cells/cytology, Dendritic Cells/immunology, Gene Dosage, Gene Expression Regulation, Lymph Nodes/cytology, Lymph Nodes/immunology, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Receptors, CCR7/physiology, Skin/cytology, Skin/immunology, Specific Pathogen-Free Organisms, T-Lymphocytes/immunology
Pubmed
Web of science
Création de la notice
14/09/2010 15:49
Dernière modification de la notice
03/03/2018 22:38
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