Cysteinyl leukotrienes modulate angiotensin II constrictor effects on aortas from streptozotocin-induced diabetic rats

Détails

ID Serval
serval:BIB_F13E87BA036B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cysteinyl leukotrienes modulate angiotensin II constrictor effects on aortas from streptozotocin-induced diabetic rats
Périodique
Arteriosclerosis, Thrombosis, and Vascular Biology
Auteur(s)
Hardy  G., Stanke-Labesque  F., Peoc'h  M., Hakim  A., Devillier  P., Caron  F., Morel  S., Faure  P., Halimi  S., Bessard  G.
ISSN
1524-4636 (Electronic)
Statut éditorial
Publié
Date de publication
11/2001
Volume
21
Numéro
11
Pages
1751-8
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
Angiotensin II (Ang II) is a vasopressor peptide involved in the pathogenesis of cardiovascular diseases associated with diabetes mellitus. We have previously reported that the 5-lipoxygenase-derived products, particularly the cysteinyl leukotrienes (CysLTs), are involved in Ang II-induced contraction. In this study, we demonstrated that CysLTs contribute to the contraction elicited by Ang II in isolated aortas from streptozotocin-induced diabetic (SS) rats but not from insulin-treated diabetic rats, fructose-fed rats, or control rats. In an organ bath, pretreatment with the 5-lipoxygenase inhibitor (AA861, 10 micromol/L) reduced by 37.6+/-8.2% and 30.1+/-10.9% the Ang II-induced contractions in intact and endothelium-denuded aortic rings, respectively, from SS rats. In contrast, the CysLT(1) receptor antagonist (MK571, 1 micromol/L) or the dual CysLT(1)/CysLT(2) receptor antagonist (BAY-u9773, 0.1 micromol/L) did not affect Ang II-induced contraction. In addition, Ang II induced a 6.2+/-1.5-fold increase in CysLT release through the stimulation of the Ang II type 1 receptor. Furthermore, the urinary excretion of leukotriene E(4) was increased in SS rats (leukotriene E(4), 13.7+/-2.9 ng/24 h [SS rats, n=10] versus 1.5+/-0.5 ng/24 h [control rats, n=6]; P<0.0004). These data suggest the activation of the 5-lipoxygenase pathway in SS rats and the involvement of 5-lipoxygenase-derived products, particularly the CysLTs, in Ang II-induced contraction in aortas from SS rats through stimulation of CysLT receptors different from the well-characterized CysLT(1) or CysLT(2) receptor.
Mots-clé
Angiotensin II/*pharmacology Animals Aorta/drug effects/metabolism/*physiopathology Arachidonate 5-Lipoxygenase/antagonists & inhibitors Benzoquinones/pharmacology Diabetes Mellitus, Experimental/metabolism/*physiopathology/urine Insulin/pharmacology Leukotriene Antagonists/pharmacology Leukotriene E4/urine Lipoxygenase Inhibitors/pharmacology Male Organ Culture Techniques Potassium Chloride/pharmacology Propionates/pharmacology Quinolines/pharmacology Rats Rats, Wistar SRS-A/*analogs & derivatives/pharmacology/*physiology Vasoconstriction/*drug effects Vasoconstrictor Agents/*pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 10:36
Dernière modification de la notice
09/05/2019 3:18
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