Indels in V4 share sequence features with insertions and deletions during somatic hypermutation of immunoglobulins : P20-20 LB

Détails

ID Serval
serval:BIB_F13560C1279F
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Poster: résume de manière illustrée et sur une page unique les résultats d'un projet de recherche. Les résumés de poster doivent être entrés sous "Abstract" et non "Poster".
Collection
Publications
Titre
Indels in V4 share sequence features with insertions and deletions during somatic hypermutation of immunoglobulins : P20-20 LB
Titre de la conférence
AIDS Vaccine 2009
Auteur(s)
Guglietta S., Pantaleo G., Graziosi C.
Adresse
Paris, France, October 19-22, 2009
ISBN
1742-4690
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
6
Série
Retrovirology
Pages
424
Langue
anglais
Notes
Background : We have shown that gp120 V4 is characterized by indels multiples of three base pairs and with no generation of stop codons. Similar insertions and deletions have also been reported during somatic hypermutation (SHM) of immunoglobulins. In this study, we have compared sequence patterns associated to indels in V4 to those reported in immunoglobulins. Our aims were i) to assess similarities between indels in V4 and SHM; ii) to assess whether sequence features of indels in V4 could also be identified in V3 and C3.
Methods : RT-PCR amplification of an Env fragment spanning C2-C4 derived from HIV plasma RNA from 12 patients at early stage disease.
Results : In the N-terminal end of V4, indels involved duplications of a 15 mer and a 9 mer, designated as SeqA (consensus ACA ACA AAA CTG TTT) and SeqB (consensus AGT ACT TGG), respectively. In the C-terminal end, indels involved homonulceotide runs, AAT stretches and other non-specific sequences. Trinucleotides, repeats and palindromes such as those described in SHM were recognized in V4 in all the patients analyzed. No duplications analogous to SeqA and SeqB could be recognized in V3 and C3 in all the patients analyzed. Trinucleotides and palindromes were detected in both regions.
Conclusion : Indels in V4 are associated to sequence features similar to those described during SHM of immunoglobulins. The presence of structural intermediates consisting of repeats, duplications, and/or palindromes indicates that indels may be the product of a mechanism of DNA misalignment, with indels resulting from DNA strand slippage during DNA synthesis. A major consequence of this mechanism is that mutations in the gp120 regions analyzed are not random but appear to be directed by the presence of sequence motifs in the nearby DNA. Further studies are needed to elucidate the mechanism of indels in hypervariable regions of gp120.
Web of science
Création de la notice
21/12/2009 13:00
Dernière modification de la notice
03/03/2018 22:38
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