The retinoblastoma-histone deacetylase 3 complex inhibits PPARgamma and adipocyte differentiation.

Détails

ID Serval
serval:BIB_F0E2F1FD8DCE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The retinoblastoma-histone deacetylase 3 complex inhibits PPARgamma and adipocyte differentiation.
Périodique
Developmental Cell
Auteur(s)
Fajas L., Egler V., Reiter R., Hansen J., Kristiansen K., Debril M.B., Miard S., Auwerx J.
ISSN
1534-5807 (Print)
ISSN-L
1534-5807
Statut éditorial
Publié
Date de publication
2002
Volume
3
Numéro
6
Pages
903-910
Langue
anglais
Résumé
The retinoblastoma protein (RB) has previously been shown to facilitate adipocyte differentiation by inducing cell cycle arrest and enhancing the transactivation by the adipogenic CCAAT/enhancer binding proteins (C/EBP). We show here that the peroxisome proliferator-activated receptor gamma (PPARgamma), a nuclear receptor pivotal for adipogenesis, promotes adipocyte differentiation more efficiently in the absence of RB. PPARgamma and RB were shown to coimmunoprecipitate, and this PPARgamma-RB complex also contains the histone deacetylase HDAC3, thereby attenuating PPARgamma's capacity to drive gene expression and adipocyte differentiation. Dissociation of the PPARgamma-RB-HDAC3 complex by RB phosphorylation or by inhibition of HDAC activity stimulates adipocyte differentiation. These observations underscore an important function of both RB and HDAC3 in fine-tuning PPARgamma activity and adipocyte differentiation.
Mots-clé
3T3 Cells, Adipocytes/cytology, Adipocytes/drug effects, Animals, Cell Differentiation/drug effects, Cell Differentiation/physiology, Gene Expression Regulation, Enzymologic/drug effects, Gene Expression Regulation, Enzymologic/genetics, Genes, Reporter/genetics, Histone Deacetylases/metabolism, Lipoprotein Lipase/genetics, Lipoprotein Lipase/metabolism, Macromolecular Substances, Mice, Phosphorylation, Protein Binding/drug effects, Protein Binding/genetics, Protein Structure, Tertiary/genetics, RNA, Messenger/metabolism, Receptors, Cytoplasmic and Nuclear/metabolism, Recombinant Fusion Proteins/diagnostic use, Retinoblastoma Protein/deficiency, Retinoblastoma Protein/genetics, Signal Transduction/drug effects, Signal Transduction/genetics, Stem Cells/cytology, Stem Cells/drug effects, Thiazoles/pharmacology, Thiazolidinediones, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/03/2013 17:07
Dernière modification de la notice
09/05/2019 3:17
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