Proteomic and transcriptomic analysis of human CD8(+) T lymphocytes over-expressing telomerase.

Détails

ID Serval
serval:BIB_F0B170003C9A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Proteomic and transcriptomic analysis of human CD8(+) T lymphocytes over-expressing telomerase.
Périodique
Proteomics. Clinical Applications
Auteur(s)
Thadikkaran L., Menzel O., Tissot J.D., Rufer N.
ISSN
1862-8346 (Print)
ISSN-L
1862-8346
Statut éditorial
Publié
Date de publication
2007
Volume
1
Numéro
3
Pages
299-311
Langue
anglais
Résumé
Human T lymphocytes have a finite life span resulting from progressive telomere shortening that occurs at each cell division, eventually leading to chromosomal instability. It has been shown that ectopic expression of the human telomerase reverse transcriptase (hTERT) gene into various human cells results in the extension of their replicative life span, without inducing changes associated with transformation. However, it is still unclear whether cells that over-express telomerase are physiologically and biochemically indistinguishable from normal cells. To address this question, we compared the proteome of young and aged human CD8(+) T lymphocytes with that of T cells transduced with hTERT. Interestingly, we found no global changes in the protein pattern in young T cells, irrespective of telomerase expression. In contrast, several relevant proteins with differential expression patterns were observed in hTERT-transduced T cells with extended life span upon long-term culture. Altogether, our data revealed that T lymphocytes over-expressing telomerase displayed an intermediate protein pattern, sharing a similar protein expression not only with young T cells, but also with aged T cells. Finally, the results obtained from this global proteomic approach are in agreement with the overall gene transcription profiling performed on the same T-cell derived clones.
Pubmed
Web of science
Création de la notice
09/09/2011 19:58
Dernière modification de la notice
03/03/2018 22:37
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