Antibody-induced modulation of the CD3/T cell receptor complex causes T cell refractoriness by inhibiting the early metabolic steps involved in T cell activation

Détails

ID Serval
serval:BIB_F096679B24B6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Antibody-induced modulation of the CD3/T cell receptor complex causes T cell refractoriness by inhibiting the early metabolic steps involved in T cell activation
Périodique
Journal of Experimental Medicine
Auteur(s)
Pantaleo  G., Olive  D., Poggi  A., Pozzan  T., Moretta  L., Moretta  A.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
08/1987
Volume
166
Numéro
2
Pages
619-24
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Résumé
We investigated the mechanism involved in T cell unresponsiveness that follows the monoclonal antibody-induced surface modulation of the CD3-TCR complex. We determined whether modulation of CD3-TCR affected the early metabolic steps such as [Ca2+]i rise and InsP3 formation. A strong inhibition of the increase on [Ca2+]i mediated by either anti-TCR or anti-CD2 mAbs was detected. In contrast, surface modulation of CD2 molecules did not prevent the [Ca2+]i increase induced by anti-TCR mAb. Similarly, InsP3 increase was strongly reduced only after modulation of CD3-TCR complex (but not of CD2 molecules). Therefore, it appears that surface modulation of CD3-TCR complex causes T cell refractoriness by inhibiting the very early metabolic events that follow receptor-ligand interactions.
Mots-clé
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface/*immunology Calcium/metabolism Cell Line Lymphocyte Activation Receptors, Antigen, T-Cell/*immunology T-Lymphocytes/*immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:14
Dernière modification de la notice
09/05/2019 3:16
Données d'usage