Human peripheral blood leukocyte engraftment into SCID mice: critical role of CD4(+) T cells

Détails

ID Serval
serval:BIB_F060D529A465
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human peripheral blood leukocyte engraftment into SCID mice: critical role of CD4(+) T cells
Périodique
Cellular Immunology
Auteur(s)
Duchosal  M. A., Mauray  S., Ruegg  M., Trouillet  P., Vallet  V., Aarden  L., Tissot  J. D., Schapira  M.
ISSN
0008-8749 (Print)
Statut éditorial
Publié
Date de publication
07/2001
Volume
211
Numéro
1
Pages
8-20
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 10
Résumé
We examined the influence of donor T lymphocytes on human peripheral blood leukocytes (PBL) engraftment into severe combined immune deficient (SCID) mice. Mice were injected with unfractionated or subset-depleted human PBL, and treated at various times with OKT3, a cytotoxic monoclonal antibody against human CD3(+) T lymphocytes. PBL engraftment, high levels of human Ig, and high incidence of lymphoproliferative disease (lpd) were found in mice transplanted with unfractionated PBL and CD8- or CD14-depleted PBL, and in mice treated with OKT3 at distance from PBL transfer. Animals xenografted with CD3- or CD4-depleted PBL, or treated at transplantation time with OKT3, had very low levels of human Ig and did not develop lpd. PBL engraftment was minimal or absent in these animals, as determined by immunohistochemistry, dot-blot, and RT-PCR analyses. These results demonstrate that the presence of donor CD4(+) T lymphocytes at transplantation time is necessary for observing human PBL engraftment into SCID mice, an essential condition for human Ig production and lpd development.
Mots-clé
Animals Antigens, CD20/biosynthesis/genetics Antigens, CD3/biosynthesis/genetics Antigens, CD45/analysis Blood/*immunology CD4-Positive T-Lymphocytes/*immunology/transplantation Chimera Humans Immunoglobulin G/blood Immunoglobulin M/blood Interleukin-6/biosynthesis Leukocytes, Mononuclear/*transplantation Lymphocyte Depletion Lymphoproliferative Disorders/*immunology/pathology Mice Mice, SCID Muromonab-CD3/pharmacology RNA, Messenger/biosynthesis Transplantation, Heterologous
Pubmed
Web of science
Création de la notice
25/01/2008 16:34
Dernière modification de la notice
03/03/2018 22:36
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