Article: article from journal or magazin.
Measuring limits of telomere movement on nuclear envelope.
Publication types: Letter ; Research Support, Non-U.S. Gov't Publication Status: ppublish
The dynamic behavior of the decondensed chromatin can be monitored by real-time fluorescence confocal microscopy. It can be observed that different chromosomal sites enjoy different degrees of freedom during a certain period, exploring larger or smaller portions of nuclear volume. Here we measure the accessible surface for two chromosomal sites (yeast telomeres Tel3R and Tel6R) that both exhibit strong preferential association with the nuclear membrane in galactose-containing media, but differ significantly in gene activity. Telomere Tel6R, which harbors an inducible gene with high levels of transcription, explores a much larger surface than the telomere Tel3R, which is adjacent to inactive chromatin. Thus, our results distinguish two perinuclear movements characteristic of different transcriptional state, allowing for a better understanding of the correlation between activity of genes and chromatin dynamics.
Binding Sites, Biophysics/methods, Cell Nucleus/metabolism, Chromatin/chemistry, Chromatin/metabolism, Galactose/chemistry, Microscopy, Confocal, Microscopy, Fluorescence, Microscopy, Video, Models, Statistical, Nuclear Envelope/chemistry, Saccharomyces cerevisiae/metabolism, Telomere/ultrastructure, Time Factors
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