Visualization of hepatitis E virus RNA and proteins in the human liver.

Détails

ID Serval
serval:BIB_EFFF1ED90897
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Visualization of hepatitis E virus RNA and proteins in the human liver.
Périodique
Journal of hepatology
Auteur(s)
Lenggenhager D., Gouttenoire J., Malehmir M., Bawohl M., Honcharova-Biletska H., Kreutzer S., Semela D., Neuweiler J., Hürlimann S., Aepli P., Fraga M., Sahli R., Terracciano L., Rubbia-Brandt L., Müllhaupt B., Sempoux C., Moradpour D., Weber A.
ISSN
1600-0641 (Electronic)
ISSN-L
0168-8278
Statut éditorial
Publié
Date de publication
09/2017
Peer-reviewed
Oui
Volume
67
Numéro
3
Pages
471-479
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Although hepatitis E constitutes a substantial disease burden worldwide, surprisingly little is known about the localization of hepatitis E virus (HEV) in the human liver. We therefore aimed to visualize HEV RNA and proteins in situ.
A panel of 12 different antibodies against HEV open reading frame (ORF) 1-3 proteins was evaluated for immunohistochemistry (IHC) and two probes for in situ hybridization (ISH) in formalin-fixed, paraffin-embedded (FFPE) HuH7 cells transfected with HEV ORF1-3 expression vectors. IHC (and partly ISH) were then applied to Hep293TT cells replicating infectious HEV and liver specimens from patients with hepatitis E (n=20) and controls (n=134).
Whereas ORF1-3 proteins were all detectable in transfected, HEV protein-expressing cells, only ORF2 and 3 proteins were traceable in cells replicating infectious HEV. Only the ORF2-encoded capsid protein was also unequivocally detectable in liver specimens from patients with hepatitis E. IHC for ORF2 protein revealed a patchy expression in individual or grouped hepatocytes, generally stronger in chronic compared to acute hepatitis. Besides cytoplasmic and canalicular, ORF2 protein also displayed a hitherto unknown nuclear localization. Positivity for ORF2 protein in defined areas correlated with HEV RNA detection by ISH. IHC was specific and comparably sensitive as PCR for HEV RNA.
ORF2 protein can be reliably visualized in the liver of patients with hepatitis E, allowing for sensitive and specific detection of HEV in FFPE samples. Its variable subcellular distribution in individual hepatocytes of the same liver suggests a redistribution of ORF2 protein during infection and interaction with nuclear components.
The open reading frame (ORF) 2 protein can be used to visualize the hepatitis E virus (HEV) in the human liver. This enabled us to discover a hitherto unknown localization of the HEV ORF2 protein in the nucleus of hepatocytes and to develop a test for rapid histopathologic diagnosis of hepatitis E, the most common cause of acute hepatitis worldwide.
Mots-clé
Cell Line, Tumor, Hepatitis E virus/isolation & purification, Humans, Immunohistochemistry, In Situ Hybridization, Liver/virology, RNA, Viral/analysis, Tissue Array Analysis, Viral Proteins/analysis, Hepatitis, Hepatitis E virus (HEV), Histopathology, Human liver, In situ hybridization
Pubmed
Web of science
Création de la notice
20/04/2017 8:46
Dernière modification de la notice
20/08/2019 17:17
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