Identification of promising antigenic components in latent fingermark residues

Détails

ID Serval
serval:BIB_EFF267C748EE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification of promising antigenic components in latent fingermark residues
Périodique
Forensic Science International
Auteur(s)
Drapel V., Bécue A., Champod C., Margot P.
Statut éditorial
Publié
Date de publication
01/2009
Peer-reviewed
Oui
Volume
184
Numéro
1-3
Pages
47-53
Langue
anglais
Résumé
An analysis of latent fingermark residues by Sodium-Dodecyl-Sulfate PolyAcrylamide Gel Electrophoresis (SDS-PAGE) followed by silver staining allowed the detection of different proteins, from which two major bands, corresponding to proteins of 56 and 64 kDa molecular weight, could be identified. Two other bands, corresponding to proteins of 52 and 48 kDa were also visualizable along with some other weaker bands of lower molecular weights. In order to identify these proteins, three antibodies directed against human proteins were tested on western blots of fingermarks residues: anti-keratin 1 and 10 (K1/10), anti-cathepsin-D (Cat.D) and anti-dermcidin (Derm.). The corresponding antigens are known to be present in the stratum corneum of desquamating stratified epithelium (K1/10, Cat.D) and/or in eccrine sweat (Cat.D, Derm.).
The two major bands were identified as consistent with keratin 1 and 10. The pro-form and the active form of the cathepsin-D have also been identified from two other bands. Dermcidin could not be detected in the western blot.
In addition, these antibodies have been tested on latent fingermarks left on polyvinylidene fluoride (PVDF) membrane, as well as on whitened and non-whitened paper. The detection of fingermarks was successful with all three antibodies.
Mots-clé
Fingerprints , Antibodies , Stratum corneum , Eccrine sweat , Keratin , Cathepsin-D , Dermcidin
Création de la notice
27/01/2009 12:35
Dernière modification de la notice
25/05/2018 14:23
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