Stability of postmortem methemoglobin: Artifactual changes caused by storage conditions.

Détails

Ressource 1Télécharger: 29245041_pp_cover.pdf (617.22 [Ko])
Etat: Serval
Version: Author's accepted manuscript
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_EFDAFCDFAEE8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Stability of postmortem methemoglobin: Artifactual changes caused by storage conditions.
Périodique
Forensic science international
Auteur(s)
Varlet V., Ryser E., Augsburger M., Palmiere C.
ISSN
1872-6283 (Electronic)
ISSN-L
0379-0738
Statut éditorial
Publié
Date de publication
02/2018
Peer-reviewed
Oui
Volume
283
Pages
21-28
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Hemoglobin is the protein in red blood cells that carries and distributes oxygen to the body. Methemoglobinemia is a blood disorder in which an abnormal amount of methemoglobin (MetHb), a form of hemoglobin (Hb), is produced from either inadequate MetHb reductase activity or too much MetHb production or by exposure to oxidizing agents. This could lead to anoxia and death if it is not treated. However, this parameter has not been investigated as a valid post-mortem indicator because random MetHb levels have been observed in various studies: MetHb increases can be observed due to autoxidation during storage, and MetHb decreases can be observed due to MetHb reductase or microbial activity in post-mortem samples. MetHb variations can also come from the blood state and can interfere in the optical measurements of MetHb. We have studied the post-mortem MetHb concentrations according to various storage conditions. Based on our results, both the post-mortem delay and the delay before analysis should be reduced whenever possible to avoid changes in MetHb. If the analysis is delayed for a short period of time (two weeks), the blood sample taken at autopsy should not be frozen but collected in EDTA preservative and stored under refrigeration (4-6°C) until analysis. If the analysis is delayed for a longer period (more than two weeks), the blood sample should be frozen with cryoprotectant at -80°C or -196°C.
Mots-clé
Artifacts, Cryopreservation, Edetic Acid, Forensic Medicine, Humans, Methemoglobin/chemistry, Postmortem Changes, Specimen Handling/methods, Hemoglobin, Methemoglobin, Postmortem
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/12/2017 10:15
Dernière modification de la notice
13/07/2019 15:53
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