Nucleotide sequence of AKV murine leukemia virus.

Détails

ID Serval
serval:BIB_EFD85979F7BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nucleotide sequence of AKV murine leukemia virus.
Périodique
Journal of Virology
Auteur(s)
Herr W.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
1984
Volume
49
Numéro
2
Pages
471-478
Langue
anglais
Résumé
AKV is an endogenous, ecotropic murine leukemia virus that serves as one of the parents of the recombinant; oncogenic mink cell focus-forming viruses that arise in preleukemic AKR mice. I report the 8,374-nucleotide-long sequence of AKV, as determined from the infectious molecular clone AKR-623. The 5'-leader sequence of AKV extends to nucleotide 639, after which lies a long open reading frame encoding the gag and pol gene products. The reading frame is interrupted by a single amber codon separating the gag and pol genes. The pol gene overlaps the env gene within the 3' region of the AKV genome. The nucleotide sequence of the 5' region of AKV reveals the following features. (i) The 5'-leader sequence lacks any AUG codon to initiate translation of gPr80gag, suggesting that gPr80gag is not required for the replication of AKV. (ii) A short portion of the leader region diverges in sequence from the closely related Moloney murine leukemia virus and appears to be related to a sequence highly repeated in eucaryotic genomes. (iii) As in Moloney murine leukemia virus, there is a potential RNA secondary structure flanking the amber codon that separates the gag and pol genes. This structure might function as a regulatory protein binding site that controls the relative levels of synthesis of the gag and pol precursors. The nucleotide sequence of the 3' region of AKV is compared with sequences reported previously from both infectious and noninfectious molecular clones of AKV.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Restriction Enzymes, DNA, Viral/genetics, Genes, Viral, Leukemia Virus, Murine/genetics, Mice, Mice, Inbred AKR, Nucleic Acid Conformation, Viral Proteins/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
03/03/2018 22:35
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