Functional GacS in Pseudomonas DSS73 prevents digestion by Caenorhabditis elegans and protects the nematode from killer flagellates.

Détails

ID Serval
serval:BIB_EFA5D6078768
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Functional GacS in Pseudomonas DSS73 prevents digestion by Caenorhabditis elegans and protects the nematode from killer flagellates.
Périodique
The ISME journal
Auteur(s)
Bjørnlund L., Rønn R., Péchy-Tarr M., Maurhofer M., Keel C., Nybroe O.
ISSN
1751-7370[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
3
Numéro
7
Pages
770-779
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The success of biocontrol bacteria in soil depends in part on their ability to escape predation. We explored the interactions between Pseudomonas strain DSS73 and two predators, the nematode Caenorhabditis elegans and the flagellate Cercomonas sp. Growth of the nematode in liquid culture was arrested when it was feeding on DSS73 or a DSS73 mutant (DSS73-15C2) unable to produce the biosurfactant amphisin, whereas a regulatory gacS mutant (DSS73-12H8) that produces no exoproducts supported fast growth of the nematode. The flagellate Cercomonas sp. was able to grow on all three strains. The biosurfactant-deficient DSS73 mutant caused severe dilation of the nematode gut. In three-species systems (DSS73, Cercomonas and C. elegans), the nematodes fed on the flagellates, which in turn grazed the bacteria and the number of C. elegans increased. The flagellates Cercomonas sp. usually kill C. elegans. However, DSS73 protected the nematodes from flagellate killing. Soil microcosms inoculated with six rhizobacteria and grazed by nematodes were colonized more efficiently by DSS73 than similar systems grazed by flagellates or without grazers. In conclusion, our results suggest that C. elegans and DSS73 mutually increase the survival of one another in complex multispecies systems and that this interaction depends on the GacS regulator.
Mots-clé
Animals, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Caenorhabditis elegans/growth & development, Caenorhabditis elegans/microbiology, Eukaryota/growth & development, Eukaryota/microbiology, Gene Deletion, Microbial Viability, Protein Kinases/genetics, Protein Kinases/metabolism, Pseudomonas/genetics, Pseudomonas/physiology, Survival Analysis, Transcription Factors/genetics, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/03/2009 16:27
Dernière modification de la notice
09/05/2019 3:13
Données d'usage