Specific developmental profiles of lysosomal and brush border enzymuria in the human.

Détails

ID Serval
serval:BIB_EFA30B4B039D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Specific developmental profiles of lysosomal and brush border enzymuria in the human.
Périodique
Biology of the Neonate
Auteur(s)
Simeoni U., Schnitzler B., Massfelder T., Hirt C., Koehl C., Hamel G., Helwig J.J., Messer J., Geisert J., Willard D.
ISSN
0006-3126 (Print)
ISSN-L
0006-3126
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
65
Numéro
1
Pages
1-6
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article Publication Status: ppublish
Résumé
Various enzymatic urinary activities have been proposed to assess renal proximal tubule damage in children, including neonates. Nevertheless comprehensive knowledge on the developmental aspects of physiological enzymuria is limited, particularly with regard to lysosomal and brush border enzymuria. Urinary activities of two lysosomal enzymes, N-acetyl-beta-D-glucosaminidase (NAG) and beta-galactosidase (GAL), and of two brush border enzymes, alanine aminopeptidase (AAG) and gamma-glutamyltransferase (GGT) were comparatively investigated in normal prematures (n = 28), term neonates (n = 52), infants aged less than 2 years (n = 19) and children (n = 33), and compared to urinary excretion of beta 2-microglobulin (B2M). Enzymatic activities were assayed using either spectrophotometrical (NAG, AAP, GGT) fluorimetrical (GAL) or radioimmunological (B2M) methods, and were related to urinary creatinine excretion. Developmental profiles of both the studied lysosomal enzymes and of B2M were similarly characterized with significantly decreasing values from prematures (NAG 9.29 +/- 1.44, GAL 2.26 +/- 0.26 IU/mmol creatinine, indicated as mean +/- SEM) to term neonates (6,94 +/- 0.58 and 1.76 +/- 0.15 IU/mmol creatinine, respectively) and older infants and children. Lysosomal enzymatic urinary activities correlated linearly with a coefficient of r = 0.75, (p < 0.05), while correlations between each lysosomal enzymatic activity and B2M urinary excretion were weaker.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Acetylglucosaminidase/urine, Adolescent, Aging/urine, Aminopeptidases/urine, Antigens, CD13, Child, Child, Preschool, Creatinine/urine, Enzymes/urine, Female, Humans, Infant, Infant, Newborn, Infant, Premature/urine, Lysosomes/enzymology, Male, Microvilli/enzymology, beta 2-Microglobulin/urine, gamma-Glutamyltransferase/urine
Pubmed
Web of science
Création de la notice
22/02/2015 12:21
Dernière modification de la notice
03/03/2018 22:35
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