Nonactivated versus thrombin-activated platelets on wound healing and fibroblast-to-myofibroblast differentiation in vivo and in vitro.

Détails

ID Serval
serval:BIB_EF61ABD90634
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nonactivated versus thrombin-activated platelets on wound healing and fibroblast-to-myofibroblast differentiation in vivo and in vitro.
Périodique
Plastic and Reconstructive Surgery
Auteur(s)
Scherer S.S., Tobalem M., Vigato E., Heit Y., Modarressi A., Hinz B., Pittet B., Pietramaggiori G.
ISSN
1529-4242 (Electronic)
Statut éditorial
Publié
Date de publication
2012
Volume
129
Numéro
1
Pages
46e-54e
Langue
anglais
Résumé
Background: Platelet preparations for tissue healing are usually preactivated before application to deliver concentrated growth factors. In this study, the authors investigated the differences between nonactivated and thrombin-activated platelets in wound healing.Methods: The healing effects (i.e., wound closure, myofibroblast formation, and angiogenesis) of nonactivated and thrombin-activated platelets were compared in experimental wounds in diabetic (db/db) animals. In vitro, fibroblast phenotype and function were tested in response to platelets and activated platelets. No treatment served as a negative control.Results: Wounds treated with platelets reached 90 percent closure after 15 days, faster than activated platelets (26 days), and with higher levels of myofibroblasts and angiogenesis. In vitro, platelets enhanced cell migration and induced twofold higher myofibroblast differentiation and contraction compared with activated platelets.Conclusions: Platelets stimulate wound healing more efficiently compared with activated platelets by enhancing fibroblast differentiation and contractile function. Similar levels of growth factors may induce different biological effects when delivered "on demand" rather than in an initial bolus. (Plast. Reconstr. Surg. 129: 46e, 2012.)
Pubmed
Web of science
Création de la notice
17/02/2012 14:20
Dernière modification de la notice
03/03/2018 22:34
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