The stereochemistry of the amino acid side chain influences the inflammatory potential of muramyl dipeptide in experimental meningitis.

Détails

ID Serval
serval:BIB_EF442FB2AEC6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The stereochemistry of the amino acid side chain influences the inflammatory potential of muramyl dipeptide in experimental meningitis.
Périodique
Infection and Immunity
Auteur(s)
Cottagnoud P., Gerber C.M., Majcherczyk P.A., Acosta F., Cottagnoud M., Neftel K., Moreillon P., Täuber M.G.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Statut éditorial
Publié
Date de publication
2003
Volume
71
Numéro
6
Pages
3663-3666
Langue
anglais
Résumé
Intrathecal injections of 50 to 100 micro g of (N-acetylmuramyl-L-alanyl-D-isoglutamine) muramyl dipeptide (MDP)/rabbit dose-dependently triggered tumor necrosis factor alpha (TNF-alpha) secretion (12 to 40,000 pg/ml) preceding the influx of leukocytes in the subarachnoid space of rabbits. Intrathecal instillation of heat-killed unencapsulated R6 pneumococci produced a comparable leukocyte influx but only a minimal level of preceding TNF-alpha secretion. The stereochemistry of the first amino acid (L-alanine) of the MDP played a crucial role with regard to its inflammatory potential. Isomers harboring D-alanine in first position did not induce TNF-alpha secretion and influx of leukocytes. This stereospecificity of MDPs was also confirmed by measuring TNF-alpha release from human peripheral mononuclear blood cells stimulated in vitro. These data show that the inflammatory potential of MDPs depends on the stereochemistry of the first amino acid of the peptide side chain and suggest that intact pneumococci and MDPs induce inflammation by different pathways.
Mots-clé
Acetylmuramyl-Alanyl-Isoglutamine/chemistry, Acetylmuramyl-Alanyl-Isoglutamine/toxicity, Animals, Humans, Inflammation/etiology, Leukocytes/drug effects, Leukocytes/physiology, Meningitis/etiology, Molecular Conformation, Rabbits, Streptococcus pneumoniae/pathogenicity, Structure-Activity Relationship, Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Création de la notice
24/01/2008 14:54
Dernière modification de la notice
03/03/2018 22:34
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