Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways.

Détails

ID Serval
serval:BIB_EF409CA3600C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways.
Périodique
EMBO Journal
Auteur(s)
Gatfield D., Unterholzner L., Ciccarelli F.D., Bork P., Izaurralde E.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
2003
Volume
22
Numéro
15
Pages
3960-3970
Langue
anglais
Résumé
The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature stop codons (PTCs). In Caenorhabditis elegans, seven genes (smg1-7) playing an essential role in NMD have been identified. Only SMG2-4 (known as UPF1-3) have orthologs in Saccharomyces cerevisiae. Here we show that the Drosophila orthologs of UPF1-3, SMG1, SMG5 and SMG6 are required for the degradation of PTC-containing mRNAs, but that there is no SMG7 ortholog in this organism. In contrast, orthologs of SMG5-7 are encoded by the human genome and all three are required for NMD. In human cells, exon boundaries have been shown to play a critical role in defining PTCs. This role is mediated by components of the exon junction complex (EJC). Contrary to expectation, however, we show that the components of the EJC are dispensable for NMD in Drosophila cells. Consistently, PTC definition occurs independently of exon boundaries in Drosophila. Our findings reveal that despite conservation of the NMD machinery, different mechanisms have evolved to discriminate premature from natural stop codons in metazoa.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, DNA Primers, Drosophila/genetics, Drosophila/metabolism, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Exons, Genes, Reporter, Hydrolysis, Molecular Sequence Data, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Sequence Homology, Amino Acid
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/12/2012 12:23
Dernière modification de la notice
09/05/2019 3:12
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