Synthesis of polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae by using intermediates of fatty acid beta-oxidation.

Détails

ID Serval
serval:BIB_EF3F190A657F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Synthesis of polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae by using intermediates of fatty acid beta-oxidation.
Périodique
Applied and Environmental Microbiology
Auteur(s)
Poirier Y., Erard N., Petétot J.M.
ISSN
0099-2240 (Print)
ISSN-L
0099-2240
Statut éditorial
Publié
Date de publication
2001
Volume
67
Numéro
11
Pages
5254-5260
Langue
anglais
Résumé
Medium-chain-length polyhydroxyalkanoates (PHAs) are polyesters having properties of biodegradable thermoplastics and elastomers that are naturally produced by a variety of pseudomonads. Saccharomyces cerevisiae was transformed with the Pseudomonas aeruginosa PHAC1 synthase modified for peroxisome targeting by the addition of the carboxyl 34 amino acids from the Brassica napus isocitrate lyase. The PHAC1 gene was put under the control of the promoter of the catalase A gene. PHA synthase expression and PHA accumulation were found in recombinant S. cerevisiae growing in media containing fatty acids. PHA containing even-chain monomers from 6 to 14 carbons was found in recombinant yeast grown on oleic acid, while odd-chain monomers from 5 to 15 carbons were found in PHA from yeast grown on heptadecenoic acid. The maximum amount of PHA accumulated was 0.45% of the dry weight. Transmission electron microscopy of recombinant yeast grown on oleic acid revealed the presence of numerous PHA inclusions found within membrane-bound organelles. Together, these data show that S. cerevisiae expressing a peroxisomal PHA synthase produces PHA in the peroxisome using the 3-hydroxyacyl coenzyme A intermediates of the beta-oxidation of fatty acids present in the media. S. cerevisiae can thus be used as a powerful model system to learn how fatty acid metabolism can be modified in order to synthesize high amounts of PHA in eukaryotes, including plants.
Mots-clé
Acyltransferases/genetics, Acyltransferases/metabolism, Blotting, Western, Fatty Acids/metabolism, Inclusion Bodies/ultrastructure, Microscopy, Electron, Oxidation-Reduction, Peroxisomes/enzymology, Polyesters/metabolism, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Recombination, Genetic, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 20:42
Dernière modification de la notice
03/03/2018 22:34
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