Isolation and complete structure of the lymphocyte serine protease granzyme G, a novel member of the granzyme multigene family in murine cytolytic T lymphocytes. Evolutionary origin of lymphocyte proteases

Détails

ID Serval
serval:BIB_EF32043D4CF2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation and complete structure of the lymphocyte serine protease granzyme G, a novel member of the granzyme multigene family in murine cytolytic T lymphocytes. Evolutionary origin of lymphocyte proteases
Périodique
Biochemistry
Auteur(s)
Jenne  D. E., Masson  D., Zimmer  M., Haefliger  J. A., Li  W. H., Tschopp  J.
ISSN
0006-2960 (Print)
Statut éditorial
Publié
Date de publication
09/1989
Volume
28
Numéro
19
Pages
7953-61
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 19
Résumé
A cDNA clone that is closely related to the granule-associated serine proteases of cytolytic T lymphocytes (CTL), called granzymes A-F, was isolated from a CTL expression library. The encoded serine protease, granzyme G, shows 70%-89% nucleotide identities to the granzymes C-F and, like those, consists of 228 amino acids preceded by the short propeptide Glu-Glu and a 18 residue long signal peptide. Granzyme G was identified by amino-terminal sequence analysis as a correctly processed and sorted protein stored in lysosome-like granules. The phylogenetic history of the granzyme multigene family was reconstructed by two tree-making methods and by Southern blot analyses of human, rat, and mouse DNA. Our results indicate differences in the evolutionary pathway between these species. The murine granzymes C-G descended from a progenitor present at the time of mammalian radiation. Granzyme C branched off first after the primate-rodent split and was involved in a recombination event with granzyme B before the rat-mouse divergence. Granzymes D and E have diverged after the mouse-rat speciation. However, no experimental evidence for the existence of a granzyme C-D-E-F-G equivalent was found in humans, and loss of the ancestral gene in the primate lineage is discussed. In view of the species differences in the number of granzyme gene copies during recent evolution, we propose that the murine granzymes B-G play several distinct roles in CTL-mediated effector functions as a response to quite recent changes of the biochemical environment.
Mots-clé
Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular Cytosol/analysis DNA/analysis *Evolution Humans Mice Molecular Sequence Data *Multigene Family Phylogeny Rats Rats, Inbred Strains Serine Endopeptidases/*genetics/isolation & purification Species Specificity T-Lymphocytes/*analysis
Pubmed
Web of science
Création de la notice
24/01/2008 16:18
Dernière modification de la notice
03/03/2018 22:34
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