Embryonic gene expression of Coregonus palaea (whitefish) under pathogen stress as analyzed by high-throughput RNA-sequencing.

Détails

ID Serval
serval:BIB_EF118418A898
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Embryonic gene expression of Coregonus palaea (whitefish) under pathogen stress as analyzed by high-throughput RNA-sequencing.
Périodique
Fish and Shellfish Immunology
Auteur(s)
Wilkins L.G., Clark E.S., Farinelli L., Wedekind C., Fumagalli L.
ISSN
1095-9947 (Electronic)
ISSN-L
1050-4648
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
47
Numéro
1
Pages
130-140
Langue
anglais
Résumé
Most fishes produce free-living embryos that are exposed to environmental stressors immediately following fertilization, including pathogenic microorganisms. Initial immune protection of embryos involves the chorion, as a protective barrier, and maternally-allocated antimicrobial compounds. At later developmental stages, host-genetic effects influence susceptibility and tolerance, suggesting a direct interaction between embryo genes and pathogens. So far, only a few host genes could be identified that correlate with embryonic survival under pathogen stress in salmonids. Here, we utilized high-throughput RNA-sequencing in order to describe the transcriptional response of a non-model fish, the Alpine whitefish Coregonus palaea, to infection, both in terms of host genes that are likely manipulated by the pathogen, and those involved in an early putative immune response. Embryos were produced in vitro, raised individually, and exposed at the late-eyed stage to a virulent strain of the opportunistic fish pathogen Pseudomonas fluorescens. The pseudomonad increased embryonic mortality and affected gene expression substantially. For example, essential, upregulated metabolic pathways in embryos under pathogen stress included ion binding pathways, aminoacyl-tRNA-biosynthesis, and the production of arginine and proline, most probably mediated by the pathogen for its proliferation. Most prominently downregulated transcripts comprised the biosynthesis of unsaturated fatty acids, the citrate cycle, and various isoforms of b-cell transcription factors. These factors have been shown to play a significant role in host blood cell differentiation and renewal. With regard to specific immune functions, differentially expressed transcripts mapped to the complement cascade, MHC class I and II, TNF-alpha, and T-cell differentiation proteins. The results of this study reveal insights into how P. fluorescens impairs the development of whitefish embryos and set a foundation for future studies investigating host pathogen interactions in fish embryos.
Mots-clé
Adaptive Immunity, Animals, Cytokines/genetics, Cytokines/metabolism, Embryo, Nonmammalian/immunology, Embryo, Nonmammalian/metabolism, Fish Diseases/genetics, Fish Diseases/immunology, Fish Proteins/genetics, Fish Proteins/metabolism, Gene Expression Regulation, High-Throughput Nucleotide Sequencing/veterinary, Pseudomonas Infections/genetics, Pseudomonas Infections/immunology, Pseudomonas fluorescens/physiology, Salmonidae, Sequence Analysis, DNA, Transcriptome
Pubmed
Web of science
Création de la notice
28/08/2015 13:32
Dernière modification de la notice
20/08/2019 16:16
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